吉林大学学报（医学版）Issue(3)：519-524,6.DOI:10.13481/j.1671-587x.20140311
红芪多糖对糖尿病肾病db／db小鼠肾功能和肾脏组织中GluT-1 mRNA和蛋白表达水平的影响
Influence of hedysaryum polysaccharide in kidney function and expressions of Glut-1 mRNA and protein in kidney tissue of db/db mice with diabetic nephropathy
摘要
Abstract
Objective To investigate the influence of hedysaryum polysaccharide (HPS)in the kidney function and expressions of Glut-1 mRNA and protein in kidney tissue of db/db mice with diabetic nephropathy (DN)and to elucidate its possible action mechanism.Methods 10 db/m mice were taken as normal control group(n=10);50 fueling animal model db/db mice with DN were randomly divided into model group,enalapril group and the low, middle and high doses of HPS groups(n=10).The mice in noral control group and model group were given physioloical saline by gavege;and the mice in the other groups were respectively given 10 mg·kg-1 ·d-1 enalapril, 100,200 and 400 mg·kg-1 ·d-1 HPS by gavage;lasted 8 weeks.Picric acid method was used to determine the serum creatinine(SCr)level of the mice,enzyme coupling rate method was used to determine the blood urea nitrogen (BUN)level,ELISA method was used to determine the urinary microalbumin(UMALB)level,RT-PCR method was performed to detect the expression of Glut-1 mRNA, and Western blotting and immunohistochemical methods were used to detect the expression of Glut-1 protein.Results Compared with model group,the levels of SCr, BUN, UMALB, the mRNA and protein of Glut-1 expressions were decreased, especially in 400 mg·kg-1 ·d-1 HPS and enalapril groups(P<0.01).The HE and Masson staining results showed that less inflammatory cells infiltration in glomerular of the mice were found, capillary lumens were unobstructed, and the collagen deposition was not obvious in 400 mg·kg-1 ·d-1 group.Conclusion HPS could improve the kidney function of the db/db mice and inhibit the Glut-1 mRNA and protein expressions obviously, which indicates that HPS could delay the development of DN by inhibiting the Glut-1 expression in the glomerular mesangial cell membrane.关键词
红芪多糖/糖尿病肾病/葡萄糖转运蛋白-1/db/db小鼠Key words
hedysaryum polysaccharide/diabetic nephropathy/glucose tansporter protein-1/db/db mice分类
医药卫生引用本文复制引用
金智生,朱真灵,魏玉娇,郑丽红,关燕,齐雪艳..红芪多糖对糖尿病肾病db／db小鼠肾功能和肾脏组织中GluT-1 mRNA和蛋白表达水平的影响[J].吉林大学学报（医学版）,2014,(3):519-524,6.基金项目
国家自然科学基金资助课题 （）
