吉林大学学报（医学版）Issue(3)：593-597,5.DOI:10.13481/j.1671-587x.20140326
硫化氢对高肺血流性肺动脉高压大鼠平滑肌细胞线粒体损伤的保护作用及其机制
Protective effect of hydrogen sulfide on mitochondrial damage of smooth muscle cells of rats with high pulmonary blood flow pulmonary hypertension and its mechanism
摘要
Abstract
Objective To study the influence of hydrogen sulfide (H2 S)on the mitochondrial function of pulmonary artery smooth muscle cells of the rats with high pulmonary blood flow pulmonary hypertension, and to clarify the function and its mechanism in the occurrence of high pulmonary blood flow pulmonary hypertension.Methods A total of 27 rats were randomly divided sham operation group,operation group,and operation+sodium hydrosulfide (NaHS)group (n=9).The pulmonary hypertension rat model was built by left lung resection.After fed for 35 d, the mean pulmonary artery pressure (mPAP),ratio of right ventricle/body weight (RV/BW),and ratio of right ventricle/(left ventricle+septum)[RV/(LV + S)]of the rats in three groups were measured.The plasma H2 S levels and the CSE activities in pulmonary artery tissue of the rats were detected;the activities of total mitochondrial ATP enzyme,superoxide dismutase (SOD),glutathione peroxidase (GSH-Px)and malondialdehyde (MDA)levels were determined;the ultrastructure of pulmonary artery smooth muscle mitochondria was observed through transmission electron microscope.Results Compared with sham operation group,the plasma H2 S level and the CSE activity in pulmonary artery tissue of the rats in operation group were decreased(P<0.01);the mitochondrial membrane of pulmonary tissue was swelling, and the mitochondrial activity was decreased (P<0.01);the mitochondrial ATP enzyme,SOD and GSH-Px activities were significantly decreased,and the MDA level was significantly increased (P<0.01).Compared with operation group,the H2S level in plasma and the activity of CSE in pulmonary tissue of the rats in operation + NaHS group were increased (P<0.01 );the mitochondrial membrane swelling was reduced, and the vitality was restored;the ATP enzyme, GSH-Px, and SOD level in pulmonary tissue were significantly increased (P<0.01),and the MDA level was significantly reduced (P<0.01).Conclusion H2 S can enhance the activities of mitochondrial ATP enzyme,GSH-Px,and SOD,and decrease the mitochondrial lipid peroxidation level,thus it plays a protective effect on rat pulmonary artery smooth muscle.关键词
硫化氢/高肺血流/肺动脉高压/平滑肌/线粒体Key words
hydrogen sulfide/high pulmonary blood flow/pulmonary hypertension/smooth muscle/mitochondria分类
医药卫生引用本文复制引用
刘军杰,黄国雄,黎静,张星,谢露..硫化氢对高肺血流性肺动脉高压大鼠平滑肌细胞线粒体损伤的保护作用及其机制[J].吉林大学学报（医学版）,2014,(3):593-597,5.基金项目
广西壮族自治区科技厅自然科学基金资助课题 （）
