吉林大学学报（医学版）Issue(3)：607-611,5.DOI:10.13481/j.1671-587x.20140329
盐霉素对人膀胱癌5637细胞的生长抑制作用
Inhibitory effect of salinomycin on growth of human bladder cancer 5637 cells
摘要
Abstract
Objective To explore the influence of salinomycin in the growth, apoptosis and invasion of human bladder cancer 5637 cells,and to clarify its possible mechanism.Methods The human bladder cancer 5637 cells cultured invitro at logarithmic growth phase were divided into control group and different doses of salinomycin(15, 30 and 60μmol·L-1 )groups.The inhibitory rate of the growth of 5637 cells in various groups was measured by MTT assay. Flow cytometry was used to detect the apoptotic rates of 5637 cells in various groups. The invasiveness of 5637 cells was tested by Matrigel Invasion Assay.The expression levels ofβ-catenin protein in 5637 cells in various groups were determined by Western blotting method. Results Compared with control group,the inhibitory rates of growth of human bladder cancer 5637 cells in different doses of salinomycin groups were increased significantly(P<0.05);the apoptotic rates were increased(P<0.05).the number of cells passed the Matrigel was decreased(P<0.05),and the expression level ofβ-catenin protein was decreased (P<0.05).Compared with low dose of salinomycin group,the inhibitory rate of growth of 5637 cells in high dose of salinomycin group was increased(P<0.05);the apoptotic rate was increased(P<0.05),the number of cells passed the Matrigel was decreased (P < 0.05 ), and the expression levels of β-catenin protein was decreased (P<0.05). Conclusion Salinomycin can inhibit the growth of 5637 cells significantly,increase the apoptosis,and decrease the cell invasion;the inhibitory effect may act by inhibiting the Wnt/β-catenin pathway.关键词
盐霉素/膀胱肿瘤/5637细胞/Wnt/β-catenin信号传导途径Key words
salinomycin/bladder neoplasms/5637cells/Wnt/β-catenin signal transduction pathway分类
医药卫生引用本文复制引用
欧仁杰,石爱平,杨红梅,王海明,许宁..盐霉素对人膀胱癌5637细胞的生长抑制作用[J].吉林大学学报（医学版）,2014,(3):607-611,5.基金项目
吉林省卫生厅科研基金资助课题（2008Z060）；吉林大学白求恩计划B项目基金资助课题 （）
