吉林大学学报（医学版）Issue(4)：715-719,5.DOI:10.13481/j.1671-587x.20140404
三氧化二砷对肝癌HepG2细胞形成血管生成拟态的影响及其机制
Influence of arsenic trioxide in vasculogenic mimicry of HepG2 cells and its mechanism
摘要
Abstract
Objective To investigate the influence of arsenic trioxide (AS2 O3 )in the vasculogenic mimicry (VM ) of HepG2 cells, and to preliminary clarify the possible mechanism of inhibition of AS2 O3 on the VM. Methods Themean inhibitory concentration (IC50 )of AS2 O3 72 h after treatment of HepG2 cells was calculated by CCK-8 assay.The HepG2 cells were cultured on 3-D Matrigel and randomly divided into control group, 1/2 IC50 AS2 O3 group and IC50 AS2 O3 group.IPP software was used to calculate the number,length and area of VM,and the expression levels of VM-related proteins VE-cadherin and MMP-2, apoptotic-related protein caspase-3 and proliferation-related protein PCNA were detected by Western blotting method.Results The IC50 of AS2 O3 was 10μmol·L-1 72 h after treatment of HepG2 cells.The number,length and area of VM in 1/2 IC50 and IC50 AS2 O3 groups were significantly lower than those in control group (P<0.01);the number,length and area of VM in IC50 AS2 O3 group were also lower than those in 1/2 IC50 AS2 O3 group (P<0.05).Compared with control group,the expression levels of VE-cadherin and MMP-2 in 1/2 IC50 and IC50 AS2 O3 groups were decreased (P<0.05),and the expression levels of caspase-3 and PCNA had no significant change (P>0.05).Conclusion AS2 O3 can inhibit the forming of VM of HepG2 cells,which indicated that its mechanism may be related to inhibiting the expressions of VE-cadherin and MMP-2 .关键词
血管生成拟态/三氧化二砷/HepG2 细胞/肝肿瘤Key words
vasculogenic mimicry/arsenic trioxide/HepG2 cells/liver neoplasms分类
医药卫生引用本文复制引用
宋海林,王雪雯,段晶晶,周明,杨莉..三氧化二砷对肝癌HepG2细胞形成血管生成拟态的影响及其机制[J].吉林大学学报（医学版）,2014,(4):715-719,5.基金项目
国家自然科学基金资助课题 （）
