吉林大学学报（医学版）Issue(5)：958-961,4.DOI:10.13481/j.1671-587x.20140510
脑室内注射5，7-双羟色胺对内侧前额叶皮层锥体神经元5-HT1 A受体敏感性的影响
Influence of intraventricular inj ection of 5,7-drhydroxytryptamine in 5-HT1A receptor sensitivity of pyramidal neurons in medial prefrontal cortex
摘要
Abstract
Objective To explore the influence of intraventricular injection of 5, 7-drhydroxytryptamine (5, 7-DHT)in 5-HT1A receptor sensitivity of medial prefrontal cortex pyramidal neurons in the rats,and to clarity the effect of 5-HT1A receptor on the eletronic response of pyramidal neurons.Methods 36 male SD rats were randomly divided into sham operation group (n=21)and 5,7-DHT lesion group (n=15).5,7-DHT was injected intraventricularly in the rats in 5,7-DHT lesion group,and the same dose saline was injected in the rats in sham operation group.The rats in two groups were intravenously injected with different doses(0.5-128.0μg·kg-1 )of 8-CH-DPAT.The firing rate of mPFC pyramidal neurons was recorded with extracellular electrophysioological examination.The rats in two groups were intravenously injected with WAY100635,the sensitivites of the rats to 8-OH-DPAT and WAY100635 in 5, 7-DHT lesion group were observed, and compared with sham operation group.Results The different doses (0.5-128.0μg·L-1 )of 8-OH-DDAT had an excitatory-inhibitory effect on the firing rate of mPFC pyamidal neurons in sham operation group;the neurons were excited when the doses of 8-OH-DPAT were 0.5-38.0μg·kg-1 ,and the firing rates were increased(P<0.05);the neurons were inhibited when the dose of 8-OH-DPAT was 128.0μg·kg-1 ,and the firing rate was decreased.The different doses(0.5-218.0μg·L-1 )of 8-OH-DPAT inhibited the elecctronic response of pyramidal neurons of the rats in 5,7-DHT lesion group in a dose-dependent manner (df=5,F=3.44,P=0.003),and the firing rates were reduced. WAY-100635 (50μg·kg-1 )reversed completely the inhibition of 8-OH-DPAT.Conclusion The sensitivity of 5-HT1A receptor of rat mPFC pyramidal neurons can be decreased by intraventricular injection of 5,7-DHT.关键词
5,7-双羟色胺/5-HT1A受体/内侧前额叶皮层/锥体神经元/电生理学Key words
5, 7-drhydroxytryptamine/5-HT1A receptor/medial prefrontal cortex/pyramidal neurons/electrophysiology分类
医药卫生引用本文复制引用
刘彦彤,高捷,王爽..脑室内注射5，7-双羟色胺对内侧前额叶皮层锥体神经元5-HT1 A受体敏感性的影响[J].吉林大学学报（医学版）,2014,(5):958-961,4.基金项目
陕西省科技厅科研基金资助课题（2012KJXX-43）；陕西省教育厅科研基金资助课题 （）
