滨州医学院学报Issue(6)：432-433,2.
乳酸菌发酵液对结肠癌细胞LS174T体外增殖与凋亡效果的研究
Effects of lactobacillus fermentation broth on the proliferation and apoptosis of LS174 cells
摘要
Abstract
Objective To explore the effect of lactobacillus fermentation broth on the apoptosis and proliferation of LS174T cells .Methods Streptococcus thermophilus(St .) ,Lactobacillus bulgaricus (Lb .) were cultivatd and fermented .MTT colori metric method was used to determine the inhibition of LS174T cells caused by two fermentation broth ,the PBS control group was set ;the rate of apoptosis were observed by Annexin VFITUPI method and flow cytometry .Results Compared with the control group ,proliferation activity of St .and Lb .groups were all falling ,Lb .groups was significantly lower than that in the con trol group at the same time .Flow cytometry analysis showed that the rate of apoptosis of St .and Lb .groups were increased and Lb .groups was significantly higher than that in the control group at the same time .Conclusion The fermentation broth of Lb . has certain inhibitory effect on the proliferation and can promote apoptosis of colon cancer cells ,which can be expected to be ap plied in colon cancer prevention .关键词
乳酸菌/发酵液/结肠癌/细胞增殖/细胞凋亡Key words
L actobacillus/fermentation broth/colon cancer/cell proliferation/cell apoptosis分类
医药卫生引用本文复制引用
杜镇镇,张珍..乳酸菌发酵液对结肠癌细胞LS174T体外增殖与凋亡效果的研究[J].滨州医学院学报,2014,(6):432-433,2.基金项目
滨州医学院科技计划项目 （）
