草业学报Issue(3)：8-19,12.DOI:10.11686/cyxb20140302
三江源区不同退化演替阶段高寒草甸土壤酶活性和微生物群落结构的变化
Changes in the activities of soil enzymes and microbial community structure at different degradation successional stages of alpine meadows in the headwater region of Three Rivers,China
摘要
Abstract
An analysis was carried out on the soil enzyme activities and the soil microbial community structure of alpine meadows in the headwater area of the Yellow River,the Yangtze River and the Lancang River at four successional degraded stages (NS:Normal Steppe,LD:Light Degradation,MD:Moderate Degradation,HD:Heavy Degradation)using the colorimetric method and phospholipids fatty acid (PLFA).1)The species and quantity of soil microbes did not reduce with degradation of the alpine meadow and they reached the highest lev-el at MD.2)The soil microbial structure at MD was the most complex one among the four stages.3)The soil microbial diversity in the 0-10 cm layer was richer than that at 10-20 cm,which made the microbial commu-nity structure better adapted to the changes of ambient environment.4)The activities of all five enzymes de-creased significantly (P<0.05)with soil depth and the activity of alkali phosphatase decreased considerably as the alpine meadow degraded (P<0.05).The maximum activities of protease and polyphenol oxidase appeared at MD,with the minimum in NS,but the activities of invertase and urease (P>0.05)seldom changed in any of the four stages.Enzyme activities responded differently to changes of the ambient environment.Alkali phos-phatase,protease and polyphenol were more sensitive than invertase and urease.5 )There was a significant correlation between soil enzyme activities and soil microbes at successional degraded stages of the alpine mead-ow (P<0.05).Soil enzyme activities and soil microbial community structure could be comprehensive indica-tors to show the stage of succession and the degree of degradation on the alpine meadows in the headwater areas of the three rivers.关键词
高寒草甸/退化演替阶段/土壤酶活性/土壤微生物群落/磷脂脂肪酸(PLFA)Key words
alpine meadow/successional degraded stages/soil enzyme activities/soil microbial community structure/PLFA分类
农业科技引用本文复制引用
胡雷,王长庭,王根绪,马力,刘伟,向泽宇..三江源区不同退化演替阶段高寒草甸土壤酶活性和微生物群落结构的变化[J].草业学报,2014,(3):8-19,12.基金项目
西南民族大学优秀学生培养工程项目（13ZYXS81），国家自然科学基金项目（31370542），国家973计划项目（No．2007CB411504）和西南民族大学研究生创新型科研项目资助。 （）
