草业学报Issue(3)：144-151,8.DOI:10.11686/cyxb20140316
干旱胁迫对鸭茅根、叶保护酶活性、渗透物质含量及膜质过氧化作用的影响
Effects of drought stress on lipid peroxidation,osmotic adj ustment and activities of protective enzymes in the roots and leaves of orchardgrass
摘要
Abstract
To elucidate the mechanism of drought tolerance in orchardgrass,the different responses associated with lipid peroxidation,osmotic adj ustment and activity of protective enzymes in the root and leaf to drought stress of two different genotypes of orchardgrass (drought-sensitive “01998”and drought-tolerant “Baoxing”) were investigated.With an extension of drought stress,MDA content and electrolyte leakage in the root and leaf of the two orchardgrasses increased,and total soluble sugar content and free proline content also accumula-ted.At the same time,soluble protein content,SOD,CAT,POD,APX activities increased initially and then decreased.The accumulation of leaf MDA content and membrane permeability grew fast,indicating that dam-age on the blades was far more serious than on roots under constant drought stress.Accumulation of osmotic substances,leaf soluble sugar and soluble protein content were higher in shoots than in the roots,while the rel-ative increase of free amino acid was greater in the roots.This might be because the blade is the main area for sugar production,whereas the root system is closely related to the synthesis of amino acids.In addition,APX and POD activities in the roots and leaves of“Baoxing”were higher than those in“01998”under 24 d drought stress,indicating that its ability to scavenge singlet oxygen and H2 O2 was not weakened by drought stress. Drought tolerant “Baoxing”showed more active and stable peroxidation ability under drought stress.关键词
鸭茅/干旱胁迫/膜质过氧化/渗透物质积累/保护酶活性Key words
orchardgrass(Dactylis glomerata)/drought stress/lipid peroxidation/osmotic accumulation/pro-tective enzyme activities分类
农业科技引用本文复制引用
季杨,张新全,彭燕,梁小玉,黄琳凯,马啸,马迎梅..干旱胁迫对鸭茅根、叶保护酶活性、渗透物质含量及膜质过氧化作用的影响[J].草业学报,2014,(3):144-151,8.基金项目
国家现代牧草产业技术体系（CARS-35-05），教育部博导类联合基金（20105103110006，20115103110004）和国家“十二五”科技支撑计划项目（2011BAD17B03）资助。 （）
