草业学报Issue(3):262-268,7.DOI:10.11686/cyxb20140331
大蒜素对辣椒炭疽病和辣椒疫病病菌的室内抑制活性测定及田间防效研究
A study on fungicidal activity of allicin against Colletotrichum capsici and Phytophthora capsici in the laboratory and its efficacy in the field
摘要
Abstract
Allicin is a botanical antibiotic extracted from garlic.To explore fungicidal activity of allicin against Colletotrichum capsici and Phytophthora capsici in the laboratory its inhibitory effects against mycelial growth and conidial production were assayed by measuring mycelial growth rate and conidial germination.Its efficacy in the field was tested by foliar spraying.Allicin showed inhibitory effects against mycelia growth,conidial production and germination of both fungi and its activity increased with increased concentrations.The EC50 was 130.15,142.60 and 127.21μg/mL against mycelial growth,conidial production and conidial germination re-spectively of C.capsici,and 128.11,123.64 and 139.68μg/mL respectively against P.capsici.Field tests showed that the efficacies of allicin 6% EW (emulsion in water)at 600 and at 1200μg/mL against C.capsici were 83.54%-88.25% respectively and 82.85%-85.88% against P.capsici.On the third day after the third spray they were 74.59%-79.16% and 74.59%-78.01% respectively but at the fourteenth day after the third spray,they were not significantly different from that of the control [carbendazim 50% WP (wettalbe powders) at 1000μg/mL].The efficacies of allicin 6% EW at 200-600μg/mL against C.capsici and P.capsici were less than 7 6% on the third to fourteenth day after the third spray,and were significantly lower than that of the control.The recommended dosage of allicin 6% EW against C.capsici and P.capsici is 600-1200μg/mL on pepper seedlings and during the fruiting period,spraying was done 3 times at 10 d intervals.关键词
大蒜素/辣椒炭疽病菌/辣椒疫病菌/抑制作用/防治效果Key words
allicin/Colletotrichum capsici/Phytophthora capsici/inhibition/efficacy分类
农业科技引用本文复制引用
周清,李保同,汤丽梅..大蒜素对辣椒炭疽病和辣椒疫病病菌的室内抑制活性测定及田间防效研究[J].草业学报,2014,(3):262-268,7.基金项目
江西省南昌市科技支撑项目(洪财2010095)资助。 ()