草业学报Issue(4)：49-60,12.DOI:10.11686/cyxb20140406
利用表型数据构建狗牙根初级核心种质
Constructing pre-core collection of Cynodon dactylon based on phenotypic data
摘要
Abstract
In this study,the method of constructing Cynodondactylon pre-core collection was studied based on 15 quantitative and qualitative traits from 831 accessions.An optimal sampling scheme was selected from 48 strategies which consisted of sampling proportions (5%,10%,15%,20%,25%,and 30%),sampling pro-portions within group (simple proportion,logarithmic proportion,square root proportion and genetic propor-tion)and individual selection methods within group (random selection and cluster selection)evaluating with four effective parameters including mean difference percentage,variance difference percentage,coincidence rate of range and changeable rate of variation coefficient.The optimal sampling scheme was also confirmed by com-paring the accessions distribution plotted by first two principal components,correlation coefficients of traits be-tween two collections.The result indicated that 25% was the optimum sampling proportions.The square root proportion was better than simple proportion,logarithmic proportion and genetic proportion.The cluster selec-tion was more suitable than random selection.At 25%sampling proportion,using square root proportion with-in group and cluster selection method within group is the most suitable method for constructing C. dactylon pre-core collection.The result conducted by PCA analysis showed the genetic structure and variation of 831 ac-cessions was well represented by the pre-core collection.The most important phenotypic correlations between traits which may be under the control of co-adapted gene complexes were also preserved by the pre-core collec-tion.Based on the optimized sampling strategy,the pre-core collection consisting 208 accessions of C. dactylon were established and will play an important role in the utilization of C. dactylon genetic resources.关键词
狗牙根/表型数据/初级核心种质/取样策略Key words
Cynodon dactylon/phenotypic data/pre-core collection/sampling strategy分类
农业科技引用本文复制引用
郑轶琦,郭琰,房淑娟,徐亚楠,陈静波,刘建秀..利用表型数据构建狗牙根初级核心种质[J].草业学报,2014,(4):49-60,12.基金项目
国家自然科学基金（31101754，31101567）资助。 （）
