草业学报Issue(4)：229-236,8.DOI:10.11686/cyxb20140428
外源甜菜碱处理对干旱胁迫下半夏氮代谢及相关酶活性的影响
Effects of exogenous betaine on the secondary metabolites of Pinellia ternata under drought stress
摘要
Abstract
To explore the effects of exogenous betaine solution on secondary metabolites and nitrogen metabo-lism enzyme activities of Pinelliaternata in a drought environment,a water pot control experiment was used. The treatments were D (drought treatment,35%±2% moisture content),D20 (drought treatment with spray of 20 mmol/L betaine),and D40 (drought treatment with spray of 40 mmol/L betaine).Glutamate synthase (GOGAT),glutamine synthetase (GS),glutamic dehydrogenation enzyme (GDH ),nitrate,soluble protein, alkaloids,guanosine and adenosine contents of P. ternata were measured in each treatment group.As drought time increased,the GOGAT and GS activities of D40 decreased slower than those of D,and D20 ,while GDH ac-tivity initially increased but then decreased.However,the CK (control group,75%±2% moisture content) group did not change significantly.The alkaloids in the D40 group improved 6.3% (9 days),10.3% (18 days) and 12.3% (27 days),which was higher than those in the D20 .The nitrate,alkaloids and guanosine contents increased with time were higher in the D40 group than in the D20 group,although the CK group was the highest. The adenosine content tended to decrease when the drought stress time reached 27 d.At 27 d,the content of soluble protein in the D40 group was 7 .3%higher than in the D,as well as 3 .1%higher than in the D20 .The 40 mmol/L exogenous betaine solution concentrations increased nitrogen metabolism enzyme activities in P. ter-nata under drought conditions.This may accelerate the inorganic nitrogen assimilation,which is conducive to the accumulation of secondary metabolites and enhances the ability of P. ternata to resist drought stress.关键词
半夏/干旱胁迫/甜菜碱/氮代谢Key words
Pinellia ternata/drought stress/betaine/nitrogen metabolism分类
农业科技引用本文复制引用
张红敏,李姣姣,黑刚刚,曹瑞霞,周心渝,李琳琳,杨卫星,吴能表..外源甜菜碱处理对干旱胁迫下半夏氮代谢及相关酶活性的影响[J].草业学报,2014,(4):229-236,8.基金项目
国家自然科学基金项目（30500041）和重庆市科技攻关计划项目（cstc2012gg-yyjs0619）资助。 （）
