草业学报Issue(5)：143-152,10.DOI:10.11686/cyxb20140516
四川白三叶根瘤菌遗传多样性及系统发育研究
Genetic diversity and phylogeny of rhizobia isolated from white clover in Sichuan Province
摘要
Abstract
The obj ective of this study was to determine the genetic diversity and phylogeny of 6 9 isolates of sym-biotic bacteria associated with wild white clover obtained from six sites in Sichuan Province,China:Ya’an, Kangding,Luding,Xichang,Chengdu and Leshan.Polymerase chain reaction restriction fragment length poly-morphism (PCR-RFLP)and phylogenetic analyses of the 16S rDNA gene,housekeeping genes (recA,glnII andatpD),nodulation gene (nodC)and nitrogen-fixing gene (nifH)were conducted in the research.Four PCR-RFLP fingerprint patterns were identified among the 69 isolates from the 16S rDNA analysis,indicating relatively high genetic diversity.The results of phylogenetic analysis of 16S rDNA and housekeeping genes were similar;nine representative strains mainly belonged to the genusRhizobiuminAlpha-Proteobacteriawere identified.In addition,nodCandnifH genes from strains from the Rhizobium genus were successfully ampli-fied by PCR,but strains belonging to the Agrobacterium genus were not able to be amplified.All of the iso-lates could be identified by species with sixty-eight strains confirmed as white clover rhizobia.The diversity of white clover rhizobia differed at different sites,indicating the potential to increase the availability of beneficial rhizobia strains .关键词
白三叶/根瘤菌/遗传多样性/16SrDNA PCR-RFLP/持家基因/系统发育Key words
white clover (Trifoliumrepens)/rhizobia/genetic diversity/16S rDNA PCR-RFLP/housekeep-ing genes/phylogeny分类
农业科技引用本文复制引用
潘明洪,凌瑶,景文,马洪平,彭燕..四川白三叶根瘤菌遗传多样性及系统发育研究[J].草业学报,2014,(5):143-152,10.基金项目
四川省科技支撑项目（No．2013NZ0013）和十二五农村领域国家科技计划课题（No．2011BAD17B03）资助。 （）
