草业学报Issue(5)：153-160,8.DOI:10.11686/cyxb20140517
两种方法在二斑叶螨电压门控钠离子通道基因突变检测中的应用
Testing two methods to detect voltage-gated sodium channels gene mutations in Tetranychusurticae
摘要
Abstract
Target mutation produces high levels of resistance to pyrethroid insecticides in the spider mite Tet-ranychusurticae.However,as there are many techniques for detecting target mutations it would be advanta-geous to identify methods that are both economical and effective.Three mutations (L1022V,A1215D and F1538I)have been identified in the voltage-gated sodium channels (VGSCs)of T.urticae.We have tested the ability of two different methods,PCR product direct sequencing and polymerase chain reaction-restriction frag-ment length polymorphism (PCR-RFLP),to detect these VGSC gene mutations in two field strains (WW-R and LZ-R)of T.urticae.The results of PCR product direct sequencing showed that the A1215D and F1538I mutations existed in the VGSC genes of both the WW-R and LZ-R strains,but that the F1022V mutation was not detected.The A1215D mutation was located between domains IIS6 and IIIS1 of the VGSCs,while the F1538I mutation was identified in domain IIIS6.The PCR-RFLP method,however,proved able to identify only the A1215D mutation.Our testing of these two methods shows that detecting VGSC mutations in field strains of T.urticaehas the potential to develop molecular-based monitoring that can be used to manage the species’resistance to pyrethroid insecticides.关键词
二斑叶螨/甲氰菊酯/靶标突变/直接测序法/PCR-RFLP法Key words
Tetranychusurticae/fenpropathrin/target mutation/direct sequencing/PCR-RFLP分类
农业科技引用本文复制引用
杨顺义,岳秀利,王进军,沈慧敏,郭金梅,沈一凡,周兴隆..两种方法在二斑叶螨电压门控钠离子通道基因突变检测中的应用[J].草业学报,2014,(5):153-160,8.基金项目
国家自然科学基金（31260442）和公益性行业（农业）科研专项（201103020）资助。 （）
