草业学报Issue(5)：168-174,7.DOI:10.11686/cyxb20140519
海滨雀稗液泡膜H＋-PPase（PvVP1）5′端的克隆和序列分析
Clone and sequence analysis of 5’RACE of PvVP1 in Paspalumvaginatum
摘要
Abstract
A pair of degenerated primers were designed based on previously cloned homologous cDNA seg-ments.The middle segment of PvVP1 was cloned,then the PvVP1 5 ’cDNA sequence also obtained using 5 ’ RACE.The open reading frame (ORF)of this sequence was 1605 bp,encoding 534 amino acids.The codon and amino acid sequences of the 5’ends of thePvVP1 gene shared 93% and 99% similarity,respectively,with those of Zeamays.关键词
海滨雀稗/液泡膜 H+-PPase/RACE/耐盐Key words
Paspalumvaginatum/H+-PPase/RACE/salt-tolerance分类
农业科技引用本文复制引用
宋辉,南志标,蔡小宁,钟小仙,顾洪如..海滨雀稗液泡膜H＋-PPase（PvVP1）5′端的克隆和序列分析[J].草业学报,2014,(5):168-174,7.基金项目
国家“973”项目（2014CB138702）资助。 （）
