草业学报Issue(5):175-183,9.DOI:10.11686/cyxb20140520
Na+对水分胁迫下白三叶抗氧化防御和有机渗透调节物质的影响
Effects of Na+ on antioxidant defence and accumulation of osmoregulatory substances in white clover underwater deficit stress
摘要
Abstract
Effects of NaCl on relative water content,root viability,chlorophyll content,reactive oxygen spe-cies,relative membrane permeability and membrane lipid peroxidation,antioxidant defence and accumulation of osmoregulatory substances in white clover (Trifoliumrepens)under water stress induced by PEG-6000 were analyzed.Under water stress,exogenous NaCl significantly improved relative water content and root viability, and effectively reduced the decomposition of chlorophyll in leaves.The treatment of exogenous NaCl improved SOD,POD,CAT activities and the key enzymes of AsA-GSH cycle activities and maintained significantly low-er electrolyte leakage and MDA content,thereby reducing water deficit stress injury to plants.50 mmol/L NaCl also can improve the growth of white clover under normal water availability,but the accumulation of sol-uble sugar and free proline was significantly lower in the exogenous NaCl treatment under water deficit stress. The results suggested that exogenous NaCl can effectively improve water deficit stress tolerance of white clover with mechanisms including enhanced antioxidant defence,but not accumulation of more osmoregulatory sub-stances.The reason maybe that white clover is like other xerophytes which accumulate Na+ as an osmoregula-tory solute under drought stress.关键词
白三叶/水分胁迫/氯化钠/抗氧化酶/渗透调节Key words
white clover (Trifoliumrepens)/water stress/NaCl/antioxidative enzyme/osmotic adj ustment分类
农业科技引用本文复制引用
李州,王晓娟,彭丹丹,彭燕..Na+对水分胁迫下白三叶抗氧化防御和有机渗透调节物质的影响[J].草业学报,2014,(5):175-183,9.基金项目
“十二五”农村领域国家科技支撑计划课题(2011BAD17B03)和教育部“春晖计划”(Z2010090)资助。 ()