草业学报Issue(5)：345-351,7.DOI:10.11686/cyxb20140541
连续混合选择下多花黑麦草杂交群体的SSR多样性变化
The genetic variation revealed by SSR markers in crossbreeding groups of annual ryegrass after continuous mass selection
摘要
Abstract
SSR markers were used to evaluate the genetic diversity in two crossbreeding groups of annual ryegrass after 5 cycles mass selection.With the 20 primer pairs selected,a total of 217 bands were detected in the two populations and the percentage of polymorphic bands was 92.2%.In the two crossbreeding groups, 72.3% or 75.2% of the genetic diversities were estimated intra-populations and 27.7% or 24.8%,respective-ly,were estimated inter-populations.The genetic diversity of the inter-population was much more than that of the intra-population.After analysis of the bands,it was found from the amplified results that the crossbreeding groups had more bands shared by parents and progeny but fewer non parental specific bands.As mass selection proceeded in the crossbreeding groups,the percentage of polymorphic bands in populations declined as did the Shannon index and Nei’s gene diversity.It is shown that genetic diversity of each population declined under mass selection.The results of this study will provide some basic information for ryegrass crossbreeding.关键词
多花黑麦草/混合选择/SSR标记/杂交育种Key words
annual ryegrass (Loliummultiflorum)/mass selection/SSR markers/crossbreeding分类
农业科技引用本文复制引用
王绍飞,黄琳凯,张新全,马啸..连续混合选择下多花黑麦草杂交群体的SSR多样性变化[J].草业学报,2014,(5):345-351,7.基金项目
国家科技支撑计划项目（编号2011BAD17B03），国家现代牧草产业技术体系（编号CARS-35）和四川省饲草育种攻关（编号2011NZ0098-11）资助。 （）
