草业学报Issue(3)：58-69,12.DOI:10.11686/cyxb20150306
不同品系高羊茅应答高温胁迫的初级代谢产物分析
Differential metabolic responses of two tall fescue genotypes to heat stress
摘要
Abstract
A study has been undertaken to investigate the effects of heat stress on the primary metabolites of tall fescue.The heat-tolerant genotype PI 578718 and the heat-sensitive genotype PI 234881 were treated with heat stress (40℃/35℃ for 12 h/12 h)for 21 days in order to determine changes in physiological parameters and me-tabolites.The results showed that grass quality,chlorophyll content,leaf relative water content,and root ac-tivity were all higher in the heat-tolerant PI 578718 than in the heat-sensitive PI 234881,while the value of leaf electrolyte leakage was lower in PI 578718.These results indicate that PI 578718 exhibited significantly more heat tolerance than PI 234881.Polar metabolite analysis of leaf and root using gas chromatography-mass spec-trometry identified 25 heat-responsive metabolites in the two tall fescue genotypes,mainly consisting of organic acids,amino acids,sugars and sugar alcohols.Most of these metabolites showed higher accumulations in PI 578718 compared with PI 234881,especially following long-term (21 days)heat stress.The differentially accu-mulated metabolites,including a sugar alcohol (inositol),two organic acids (citric acid and hexadecanoic acid), one sugar (sucrose)and three amino acids (Val,Glu and Pro),may be positively associated with the differenti- al heat tolerance of the two genotypes.Moreover,citric acid mainly served as an antioxidant and intermediate in respiration metabolisms.Its involvement in other defense pathways may play a vital role in tall fescue adap-tation to heat stress.关键词
高羊茅/高温胁迫/生理应答/气相色谱质谱联用/初级代谢物Key words
tall fescue/heat stress/physiological response/GC-MS (gas chromatography and mass select-de-tector,MS)/metabolite profile引用本文复制引用
赵状军,胡龙兴,胡涛,傅金民..不同品系高羊茅应答高温胁迫的初级代谢产物分析[J].草业学报,2015,(3):58-69,12.基金项目
国家自然科学基金（31101563，31272194）和863项目（2011AA100209-2）资助。 （）
