草业学报Issue(4)：87-94,8.DOI:10.11686/cyxb20150410
吸胀冷害下外源 NO 对紫花苜蓿种子萌发及抗氧化性的影响
Effects of nitric oxide on Medicago sativa seed germination under imbibitional chilling
摘要
Abstract
Seeds of Medicago sativa were used to study the effects of 0.1 mmol/L sodium nitroprusside (SNP), as a source of nitric oxide,on seed germination and antioxidant capacity under imbibitional chilling at 4℃.Four treatments:control,chilling,SNP and chilling+SNP were replicated three times.The results indicated that after imbibitional chilling for 12 h and germination at 25℃ for 84 h,the vigor and germination of M.sativa seeds were increased by SNP treatment.The activities of antioxidant enzymes including superoxide dismutase (SOD)and catalase (CAT)increased 149.3% to 60.4% by SNP treatment.The radical clearance rate tended to decline with SNP treatments and chilling for 12,24 and 48 h.At 12 h chilling the radical clearance rate reached a maximum of 97.5%.Imposing exogenous nitric oxide can promote germination,enhance antioxidant enzyme activities and reduce the reactive oxygen damage in M.sativa seed.关键词
一氧化氮/紫花苜蓿/吸胀冷害/抗氧化性Key words
nitric oxide/Medicago sativa seed/chilling imbibition/antioxidant引用本文复制引用
赵萌,魏小红..吸胀冷害下外源 NO 对紫花苜蓿种子萌发及抗氧化性的影响[J].草业学报,2015,(4):87-94,8.基金项目
甘肃省自然基金（145RJZA196）资助。 （）
