草业学报Issue(4)：148-156,9.DOI:10.11686/cyxb20150418
亚精胺对水分胁迫下白三叶脯氨酸代谢、抗氧化酶活性及其基因表达的影响
Effects of spermidine on proline metabolism,antioxidant enzyme activity and gene expression in white clover leaves under water stress
摘要
Abstract
The effects of spermidine (Spd)on relative water content,reactive oxygen,membrane lipid preoxi-dation,antioxidant enzyme activity and proline metabolism were studied in white clover (Trifolium repens ) leaves,cultivar ‘Ladino’.The expression patterns of four different antioxidant enzyme genes were analyzed u-sing semi-quantitative RT-PCR under different water stress.Under water stress,relative water content grad-ually declined whereas reactive oxygen and malondialdehyde (MDA)content increased.Exogenous Spd signifi-cantly improved relative water content and effectively reduced the accumulation of reactive oxygen and MDA. Exogenous Spd changed antioxidant activities and gene expression pattern under different water stress regimes and increased expression of superoxide dismutase (SOD),catalase (CAT),peroxidase (POD)and ascorbate peroxidase (APX)genes to improve antioxidant enzyme activities,reducing water stress injury.Exogenous Spd accelerated proline catabolism and biosynthesis from the glutamate pathway during water stress although it had no effect on the ornithine pathway of proline biosynthesis.The results suggested that exogenous Spd can effec-tively improve water stress tolerance of white clover associated with the improving antioxidant enzyme activi-ties,enhancing expression of genes encoding antioxidant enzymes and accelerating proline metabolism.关键词
白三叶/亚精胺/水分胁迫/抗氧化酶/基因表达/脯氨酸Key words
white clover (Trifolium repens )/spermidine/water stress/antioxidant enzyme/gene expression/proline引用本文复制引用
李州,彭燕..亚精胺对水分胁迫下白三叶脯氨酸代谢、抗氧化酶活性及其基因表达的影响[J].草业学报,2015,(4):148-156,9.基金项目
国家自然科学基金项目（NSFC31372371）和十二五农村领域国家科技计划课题（2011BAD17B03）资助。 （）
