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四个抗草甘膦基因的抗性比较

刘健 肖雅文 芦佳 吴忠义 张中保 徐杰 姚磊

草业学报Issue(5):159-166,8.
草业学报Issue(5):159-166,8.DOI:10.11686/cyxb20150519

四个抗草甘膦基因的抗性比较

Comparison of four glyphosate tolerance genes

刘健 1肖雅文 2芦佳 3吴忠义 1张中保 2徐杰 3姚磊3

作者信息

  • 1. 内蒙古师范大学生命科学与技术学院,内蒙古 呼和浩特 010022
  • 2. 北京市农林科学院农业生物技术研究中心,北京 100097
  • 3. 北京市农林科学院农业生物技术研究中心,北京 100097
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摘要

Abstract

Glyphosate tolerance is an important agronomic trait in modern agriculture.In order to determine the best glyphosate tolerance gene for plant selectable markers we compared four glyphosate tolerance genes,cp4-epsps ,GR79-epsps ,gat4621 and GAT,in Arabidopsis .Four transgenic plants harboring each gene were trea-ted with four different rates of glyphosate.The results showed that the resistance of plants with GAT gene was significantly higher than plants with the epsps gene.Plants with the gat4621 gene had the highest survival rate at all herbicide rates.The plants with the domestic GAT gene also had strong resistance.Reproductive devel-opment of Arabidopsis plants with the GAT gene was suppressed at higher glyphosate application rates.It was concluded that the GAT gene was more suitable as a selectable marker gene.

关键词

比较/草甘膦/抗性/基因

Key words

comparison/glyphosate/tolerance/gene

引用本文复制引用

刘健,肖雅文,芦佳,吴忠义,张中保,徐杰,姚磊..四个抗草甘膦基因的抗性比较[J].草业学报,2015,(5):159-166,8.

基金项目

北京市农林科学院科技创新能力建设专项(No.KJCX201102003)资助。 ()

草业学报

OA北大核心CSCDCSTPCD

1004-5759

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