草业学报Issue(5):159-166,8.DOI:10.11686/cyxb20150519
四个抗草甘膦基因的抗性比较
Comparison of four glyphosate tolerance genes
摘要
Abstract
Glyphosate tolerance is an important agronomic trait in modern agriculture.In order to determine the best glyphosate tolerance gene for plant selectable markers we compared four glyphosate tolerance genes,cp4-epsps ,GR79-epsps ,gat4621 and GAT,in Arabidopsis .Four transgenic plants harboring each gene were trea-ted with four different rates of glyphosate.The results showed that the resistance of plants with GAT gene was significantly higher than plants with the epsps gene.Plants with the gat4621 gene had the highest survival rate at all herbicide rates.The plants with the domestic GAT gene also had strong resistance.Reproductive devel-opment of Arabidopsis plants with the GAT gene was suppressed at higher glyphosate application rates.It was concluded that the GAT gene was more suitable as a selectable marker gene.关键词
比较/草甘膦/抗性/基因Key words
comparison/glyphosate/tolerance/gene引用本文复制引用
刘健,肖雅文,芦佳,吴忠义,张中保,徐杰,姚磊..四个抗草甘膦基因的抗性比较[J].草业学报,2015,(5):159-166,8.基金项目
北京市农林科学院科技创新能力建设专项(No.KJCX201102003)资助。 ()