草业学报Issue(5)：175-181,7.DOI:10.11686/cyxb20150521
胶孢炭疽菌侵染柱花草叶片的显微观察
Microscopic observation ofStylosanthese infected by Colletotrichum gloeosporioides
摘要
Abstract
In order to study the disease progress of Stylo anthrocnose and the inoculated plants reaction,the 6 weeks old of Stylosanthese guianensis cv.Reyan2 inoculated by the suspension liquid of Colletotrichum gloeos-porioides spores.Microscopic observations of the leaves revealed the infecting process and counting the disease index through plant symptoms.Spores germinated and a few spores formed appressorium at 4 h.Most of spores germinated at 6 h,and a large quantity of geminating spores formed appressorium at 12 h and began to form penetration pegs.The inoculated spores began to produce hyphae at 36 h,a lot of hyphae formed and staggered into a mesh on the surface of the leave at 48 h.The geminating spores began to form conidiophores at 60 h.Numerous of new spores began to produce at 72 h.Parts of inoculated spores have finished disease cycle in 72 h.In the same time,plants appeared disease symptom gradually:leaves color became yellow,the host cell membrane rupture at 24 h,brown necrosis appeared at 48 h,then dead line,sclerotinite,mycelium appeared on the leaves surface at 72 h.The host cell began to collapse at 96 h.Disease index is 54.52 at 8 days,55% of inoculated plants dead at 11 days.关键词
柱花草/柱花草炭疽菌/侵染/显微结构/病害症状Key words
Stylosanthese/Stylo anthrocnose/infection/microstructure/desease symptom引用本文复制引用
杨茂霞,林国彪,陈彩虹,王荟,罗丽娟..胶孢炭疽菌侵染柱花草叶片的显微观察[J].草业学报,2015,(5):175-181,7.基金项目
国家自然科学基金（31360575）和国家牧草产业技术体系热带牧草育种（CARS-35-03）资助。 （）
