草业学报Issue(6)：177-187,11.DOI:10.11686/cyxb2014452
甘肃红豆草无色花青素还原酶 LAR基因的克隆和表达分析
Cloning and Expression Analysis of a Leucoanthocyanidin Reductase (LAR)Gene from Onobrychis viciifolia cv.Gansu
摘要
Abstract
A leucoanthocyanidin reductase (LAR)gene was cloned using RT-PCR from Onobrychis viciifolia (sainfoin)cv.Gansu,and the level of LAR gene expression was analyzed in different plant organs.The LAR gene sequence from O.viciifolia cv.Gansu shared a high level of similarity (98.34% homology)with an O. viciifolia LAR gene in GenBank (accession No:HM152980).However,the coding sequence of amino acids is obviously different from those in other genera of the Fabaceae.The open reading frame (ORF)comprised 1089 bp,encoding 362 amino acid residues.The sequence of the LAR gene from the present study was submitted to GenBank with the accession number KP013623.LAR expression differed between plant organs of O.viciifolia cv.Gansu.LAR expression was highest in leaves,followed by flowers and fruit,and was lowest in stem tis-sues,which corresponded to concentration of condensed tannins in different organs of O.viciifolia cv.Gansu. It is inferred that LAR gene expression is in some way involved in condensed tannin accumulation in O.viciifoliacv.Gansu.These results provide fundamental information and a method for exploring the regulation mecha-nism of condensed tannin accumulation.关键词
甘肃红豆草/RT-PCR/无色花青素还原酶/克隆与表达Key words
Onobrychis viciifolia cv.Gansu/RT-PCR/leucoanthocyanidin reductase/cloning and expression引用本文复制引用
陈春艳,马晖玲,董文科..甘肃红豆草无色花青素还原酶 LAR基因的克隆和表达分析[J].草业学报,2015,(6):177-187,11.基金项目
甘肃省科技厅科技支撑计划项目（1104NKCA087）和国家自然科学基金项目（31200299）资助。 （）
