草业学报Issue(7)：89-96,8.DOI:10.11686/cyxb2014502
外源钙和赤霉素对干旱胁迫下苜蓿幼苗生理特性的影响
Effects of calcium (CaCl2),GA3 and complex liquid on the physiological characteris-tics of alfalfa seedlings under drought stress
摘要
Abstract
This study aimed to investigate the effects of calcium (CaCl2 )and GA3 on alfalfa seedlings’physio-logical characteristics under drought stress and to discover appropriate concentrations for alleviating plant dam-age.Alfalfa seedlings were cultured in sand.The tested concentrations for CaCl2 were 0,5,10 and 20 mmol/L;for GA3 they were 0,50,100 and 150 mg/L,also tested was a complex liquid of 10 mmol/L CaCl2 with 100 mg/L GA3 (CaCl2 :GA3 ).These four treatments (T1 ∶1 ,T1 ∶2 ,T2 ∶1 and Z1 ∶1 respectively)were inves-tigated under conditions of PEG-6000 drought stress.Results indicated that chlorophyll relative content de-creased under stress,while relative electrolytic leakage and MDA,Pro,SOD and POD contents increased sig-nificantly.Appropriate concentrations of CaCl2 ,GA3 and complex liquid could alleviate the degradation of seedling chlorophyll and reduce the relative conductivity and accumulation of MDA that protect film stability and reduce the content of osmotic regulation substances.They can also maintain higher SOD and POD activity and significantly improve alfalfa seedling resistance to drought stress.Using the method of subordinate func-tion,it was found that 10 mmol/L CaCl2 ,100 mg/L GA3 and CaCl2 +GA3 T1 ∶1 performed better than 10 mmol/L CaCl2 and 100 mg/L GA3 .The study concluded that the relative drought relief effect of the treatments from strong to weak was T1 ∶1 >10 mmol/L CaCl2 >T2 ∶1 >CK1 >T1 ∶2 >100 mg/L GA3 >Z1 ∶1 >CK2 .关键词
苜蓿幼苗/CaCl2/GA3/复合液/干旱胁迫Key words
alfalfa seedling/CaCl2/GA3/complex liquid/drought stress引用本文复制引用
郭郁频,任永霞,刘贵河,曹春梅,闫利军..外源钙和赤霉素对干旱胁迫下苜蓿幼苗生理特性的影响[J].草业学报,2015,(7):89-96,8.基金项目
学院创新人才培育项目（CXRC1313），校级课题（2011015）和公益性行业（农业）科研专项经费（201203024）资助。 （）
