川北医学院学报Issue(4)：351-354,4.DOI:10.3969/j.issn.1005-3697.2014.04.08
金黄地鼠胰腺癌动物模型的建立
Establishment of animal model of pancreatic carcinoma in Syrian golden hamsters
摘要
Abstract
Objective:To establish the N-Nitrosobis (2-oxopropyl)amine(BOP)-induced animal model of pancreatic cancer in ham-sters. Methods:120 female Syrian golden hamsters were randomly divided into experimental group (80) and control group (40). The hamsters of experimental group were subcutaneously injected with 10mg/kg body weight BOP,once a week for 7 weeks;control group were injected with normal saline. 10 hamsters in experimental group and 5 in control group were sacrificed randomly at the 11th-week after the first injection and thereafter at 2-week intervals respectively. Histopathologic examinations were performed. Results:Jaundice, malnutrition and metastasis were found in some animals of experimental group. The average weight was lighter than control group,and the difference was statistically significant (P<0. 05). 23 pancreatic cancers were found by naked eyes. One case showed 3 pancreatic tumors. 3 cases showed 2 tumors,and the others were single. Among them,tumor diameter is smaller than 5mm while equal to or larger than 5mm was found in 7 and 16 respectively. The median tumor diameter,volume and weight was 6. 8mm,178. 71 mm3 and 0. 32 g re-spectively. Pathologic findings of 9 cases of pancreatic carcinoma are in situ. 22 cases of invasive carcinoma were all pancreatic ductal adenocarcinomas. All tumor-bearing hamsters were associated with different degrees of atypical ductal hyperplasia. Atypical ductal hyperplasia were found in toto animals 19 weeks later. Totally 65 cases of atypical ductal hyperplasia were found in the experimental group. The overall tumor-generating rate was 81. 3%(65/80). Conclusion:BOP successfully induced PDAs in Syrian golden hamsters. The model establishment method is simple and easy to copy. Histopathologic findings are similar to human pancreatic cancer. It is suggested that it’s an ideal animal model of pancreatic cancer for in vivo studies.关键词
叙利亚金黄地鼠/诱导/胰腺癌/动物模型Key words
Syrian gold hamster/Induction/Pancreatic cancer/Animal model分类
医药卫生引用本文复制引用
马青松,张小明,沈成义,吴泽珊,蒲宇,武超颖,杨正伟..金黄地鼠胰腺癌动物模型的建立[J].川北医学院学报,2014,(4):351-354,4.基金项目
国家自然科学基金(30770612) （30770612）
四川省青年基金(08ZQ26-048) （08ZQ26-048）
四川省教育厅重大科技培育项目(07ZZ010) （07ZZ010）
