川北医学院学报Issue(5)：416-420,5.DOI:10.3969/j.issn.1005-3697.2014.05.01
虫草菌丝对 IL-1β诱导 MIN6细胞凋亡的保护作用与机制的实验研究
Protective effects of cordyceps sinensis on MIN6 cells apoptosis induced by IL-1βand experimental research on the mechanism
摘要
Abstract
Objective:Observe the influence of cordyceps sinensis( CS) on MIN6 cell apoptosis induced by IL-1βand its relationship with reactive oxygen species and UCP-2 in order to investigate the protective effects and mechanism of islet cells. Methods:The experi-ment groups were divided into normal control group,IL-1 beta group,IL-1β+CS low concentration group and IL-1β+CS high concen-tration group. The cell activity was tested by CCK8. The basal insulin of MIN6 cells and acute insulin response irritated by glucose were tested by Method Elisa. The cell apoptosis was detected by Hochest dyeing and flow cytometry. The reactive oxygen species were detec-ted by chemical fluorescence method. The expression of uncoupling protein 2 was detected by Western Blot. Results:Compared with the normal group, the cell activity and insulin secretion of IL-1β group reduced significantly. The cell apoptosis increased significantly, Meanwhile,the ROS activities increased and the UCP-2 decreased. After the low and high concentration CS were given,compared with the damage group,the cell activity and insulin secretion increased obviously. The apoptosis rate reduced significantly. The UCP-2 con-tent increased and the ROS activity decreased at the same time. Conclusion:CS would increase the cell activity and insulin secretion. It reduces the MIN6 cell apoptosis induced by IL-1β. The mechanism may be related with the increasing of expression of UCP-2 and the reduction of ROS activity.关键词
虫草菌丝/白介素-1β/胰岛细胞/细胞凋亡/活性氧/解偶联蛋白2Key words
Cordyceps sinensis(CS)/IL-1β/Islet cells/Cell apoptosis/Reactive oxygen species(ROS)/Uncoupling protein 2(UCP-2)分类
医药卫生引用本文复制引用
郑倩,刘华,曹弟勇,杨尚君,赵小蓉,米贵飞,赵雅茜,肖昊卓..虫草菌丝对 IL-1β诱导 MIN6细胞凋亡的保护作用与机制的实验研究[J].川北医学院学报,2014,(5):416-420,5.基金项目
四川省教育厅项目(11ZB159) （11ZB159）
川北医学院重点项目(CBY12-A-ZD06) （CBY12-A-ZD06）
川北医学院创新团队项目(CBY13-TD02) （CBY13-TD02）
