川北医学院学报Issue(5)：421-424,4.DOI:10.3969/j.issn.1005-3697.2014.05.02
性别差异对1型糖尿病大鼠造模的影响
Impact of gender difference in type 1 diabetic rats modeling
摘要
Abstract
Objective:To explore the difference of male and female rats in establishing diabetic model by intraperitoneally injecting a high dose streptozotocin( STZ) . Methods:Total 118 male and female SD rats( each 59 rats) were randomly divided into four groups,in-cluding male mode group(n=46),female mode group(n=46),male normal control group(n=13)and female normal control group(n=13). Model groups were induced to type 1 diabetes mellitus by intraperitoneally injecting 60 mg/kg STZ,normal control received in intraperitoneal injection with citric acid buffer. The body weight,fasting blood glucose and the morphology of the spleen ware measured and examined to validate the animal models. Results:Blood glucose of rats was markedly increased after STZ injection. And there were some other symptoms of model rats,such as polyuria,polydipsia and polyphagia,which indicated that type 1 diabetes mellitus had been induced in the models. Compared to male rats,the incidence of female did not changed significantly. Compared to diabetic male rats,the mortality of diabetic female rats decreased significantly. Conclusion:60 mg/kg establishment of male and female rats with type 1 diabe-tes model induced by STZ and there was no significant change of incidence between male rats and female rats. But the mortality of dia-betic rat was changed significantly. Therefore in the pathogenesis of diabetes and diabetic complications should be taken both male rats and female rats into account in the study.关键词
1型糖尿病/性别差异/链脲佐菌素/成模率/死亡率Key words
Type 1 diabetes mellitus/Gender difference/Streptozotocin/Incidence/Mortality rate分类
医药卫生引用本文复制引用
赵圆宇,陈科利,张露丹,马亚洪,谭敏,王艺蓉,徐彩婷,赵丰,莫琳龙,胡林均,施蕾,李生玉,卢营杰..性别差异对1型糖尿病大鼠造模的影响[J].川北医学院学报,2014,(5):421-424,4.基金项目
国家自然科学青年基金(81400806) （81400806）
四川省科技厅应用基础项目(2014JY0012) （2014JY0012）
四川省教育厅重点课题(14ZA0180) （14ZA0180）
