长春大学学报（自然科学版）Issue(2)：464-467,4.
几种废啤酒酵母细胞破壁方法的比较
Comparison of a Few Kinds of Cell-wall Breaking Methods for Waste Beer Yeast
摘要
Abstract
This study, taking waste beer yeast as material, according to the laboratory conditions and test requirements, adopts the methods of grinding,freeze thawing,microwave and ultrasonic to break waste beer yeast cell-wall. By using ultraviolet spectrophotometer to determine the content of soluble protein in supernatant, it evaluates the role of different breaking method on the release extent of cell inclusion, which provides theoretical references for the further exploration of economical and effective methods. The results show that the effects of waste beer yeast cell-wall breaking are ultrasonic﹥microwave﹥freeze thawing﹥grinding.关键词
废啤酒酵母/破壁方法/可溶性蛋白质Key words
waste beer yeast/cell-wall breaking method/soluble protein分类
轻工纺织引用本文复制引用
张莉弘,吴琼..几种废啤酒酵母细胞破壁方法的比较[J].长春大学学报（自然科学版）,2014,(2):464-467,4.基金项目
吉林省教育厅“十二五”科学技术研究项目(吉教科合字[2012]第257号) （吉教科合字[2012]第257号）
