长春大学学报（自然科学版）Issue(5)：1380-1383,4.
HLPC梯度洗脱测定抗荨胶囊中升麻素苷和5-O-甲基维斯阿米醇苷的总含量
Quantitative Determination of Cimicifugoside and 5-O-omethylvisammios-De in Anti-nettle Capsules by HPLC Gradient Elution
摘要
Abstract
Objective:To establish a high-performance liquid chromatography ( HPLC) for simultaneous determination of cimicifugoside and 5-O-methylvisammios-De in anti-nettle capsules ( radix saposhnikoviae, caltrop, periostracum cicadae, Astragalus membranaceus, angelica sinensis, dictamnus dasycarpus, etc. ). Methods:WondaSil C18-WR (4. 6 mm × 150mm, 5 μm PH 1-10) is used as the column;Mobile phase has been made gradient elution with (A) -0. 05% phosphoric acid:0~6min,40%B,6~17min,45%(B),17~30min, 75%(B), the detection wavelength of cimicifugoside and 5-O-methylvisammios-De is 254 nm, the flow speed is 1. 0mL· min-1 , the column temperature is 30℃, and the injection volume was 10 μl. Results: A better separation result is obtained by the gradient elution. There was a good linear relationship for cimicifugoside from 18. 4 to 92. 0 μg/mL r=0. 9999550(n=6) and for 5-O-methylvisammioside from 22. 0 to 110. 0μg/mL,r=0. 9996565(n=6), the average recovery rate is 100. 28 and 100. 56% respective-ly. Conclusion:The quantitative analysis of determination of cimicifugoside and 5-O-methylvisammios-De is conducted. The established method with high accuracy is specific, which can be used to determine the contents of cimicifugoside and 5-O-methylvisammios-De in anti-nettle capsules.关键词
抗荨胶囊/升麻素苷/5-O-甲基维斯阿米醇苷Key words
anti-nettle capsules/cimicifugoside/5-O-methylvisammios-De分类
医药卫生引用本文复制引用
史春山,姜宗文,姜楠,肖秋生..HLPC梯度洗脱测定抗荨胶囊中升麻素苷和5-O-甲基维斯阿米醇苷的总含量[J].长春大学学报（自然科学版）,2014,(5):1380-1383,4.基金项目
吉林省中医药管理局科技项目(2012-167) （2012-167）
