磁共振成像Issue(4)：302-308,7.DOI:10.3969/j.issn.1674-8034.2014.04.013
9.4T1H-MRS定量检测阿尔茨海默病模型小鼠海马代谢物浓度的研究
Quantitative research for metabolites by 9.4 T 1H-MRS in hippocampus of Alzheimer's disease mice
摘要
Abstract
Objective:To study Alzheimer's disease mice caused by the D-galactose and NaNO2, observe the alteration of hippocampal metabolites using in vitro 9.4 T high resolution magnetic resonance spectroscopy. Materials and Methods:Twenty kunming mice were randomly divided into two groups, model group and control group. Female to male ratio was 1:1. The mice in the model group were subcutaneouly injected with D-galactose 120 mg/(kg.d), NaNO2 90 mg/(kg.d) for 60 days and the control group with oral saline. 24 hours later after Morris water maze test, all mice were sacriifced and the right hippocampus were dissected for 1H-MRS examination. Data were collected using in vitro 9.4 T high resolution magnetic resonance spectrometer. Spectra were processed using XWINNMR and MestRe-c 4.3. Compared with the left side, HE and Bielschowsky silver impregnation and congored coloration were employed to detect and conifrm the change of brain cells. Results:Good 1H-MR spectra of perchloric acid extract from hippocampus tissue of mice were obtained. The conventional metabolites were detected and assigned. Mean concentrations of metabolites in control group and model group were, NAA:(43.63±7.67) mmol/L and (34.66±6.79) mmol/L. Cho:(53.09±4.32) mmol/L and (48.62±7.92) mmol/L. Glu:(26.87±5.46) mmol/L and (14.87±2.68) mmol/L. mI:(45.93±6.73) mmol/L and (74.09±8.09) mmol/L, the differences of two groups were statistically signiifcance (P<0.05). Results of Morris water maze behavior examination were as: the escape lateney and the movement distance percentage and time percentage were significant difference between control group and model group (P<0.05). The neurons in control group were intact and arrange tightly. In the model, pyramidal neurons either presented a densely stained shrunken appearance with minimal cytoplasm or had disappeared. Bielschowsky silver impregnation, many neuroifbrillary tangles were found, and neuropil threads are stained in model group. Congored coloration result:in the cerebral cortex and hippocampal ifelds, orange amylaceous aggradation can be seen. Conclusions:D-galactose and NaNO2 can cause the mice induce Alzheimer's disease in vivo. High resolution 1H-MRS in vitro can detect diversiifed metabolism. In the model group the hippocampal concentrations of NAA, Cho, Glu are decrease. The changing trend for mI is increase.关键词
阿尔茨海默病/海马/代谢/磁共振成像/动物,实验Key words
Alzheimer disease/Hippocampus/Metabolism/Magnetic resonance imaging/Animals laboratory分类
医药卫生引用本文复制引用
庞丽,姚建莉,陈耀文,李海红,尤克增,徐志锋,吴仁华..9.4T1H-MRS定量检测阿尔茨海默病模型小鼠海马代谢物浓度的研究[J].磁共振成像,2014,(4):302-308,7.基金项目
国家自然科学基金重点项目资助(编号30930027) （编号30930027）
