重庆医学Issue(10)：1161-1163,1167,4.DOI:10.3969/j.issn.1671-8348.2014.10.003
人β-NGF重组质粒转染GFP转基因小鼠BMSC的实验研究
Experimental study on transfection of humanβ-NGF recombinant plasmid into BMSCs of GFP transgenic mouse
摘要
Abstract
Objective To investigate the biological features of the mouse bone marrow stromal stem cells (BMSCs) transfected by humanβnerve growth factor(β-NGF) .Methods BMSCs of GFP transgenic mouse were isolated and cultured .Theβ-NGF re-combinant plasmid vectors were transferred into the cultured BMSCs by LipofectamineTM 2000 .The expression of β-NGF was detec-ted with ELISA .Results The β-NGF recombinant plasmid vectors were successfully transferred into BMSCs of GFP transgenic mouse ,the expression of β-NGF in transfected cells appeared .In addition ,the expression ofβ-NGF could effectively protect the BM-SCs which were injured in transfection process .Conclusion BMSCs of GFP transgenic mouse after transfecton with human β-NGF have the proliferation and differentiation capacity ,and possess the expression ability of β-NGF protein .关键词
神经生长因子/绿色荧光蛋白质类/Lipofectamine 2000/转染/小鼠/骨髓基质干细胞Key words
nerve growth factor/green fluorescent proteins/lipofectamine 2000/transfection/mice/bone marrow stromal stem cells引用本文复制引用
刘强和,王亮亮,王翔,王永宝,黄鑫,邓铭,刘芳贤..人β-NGF重组质粒转染GFP转基因小鼠BMSC的实验研究[J].重庆医学,2014,(10):1161-1163,1167,4.基金项目
国家自然科学基金资助项目（81060083）；广西科技厅技术研究与开发项目（桂科攻10124001B-56）。 （）
