重庆医学Issue(10)：1214-1217,4.DOI:10.3969/j.issn.1671-8348.2014.10.022
骨关节炎SD大鼠关节软骨中蛋白多糖与YKL-40的相关性
Relationship between Aggrecan and YKL-40 in articular cartilage of Sprague-Dawlay rats with osteoarthritis
摘要
Abstract
Objective To investigate the relationship between aggrecan and YKL-40 in knee articular cartilage of Sprague-Daw-lay(SD) rats with osteoarthritis (OA) .Methods Fifty-six healthy SD rats were randomly divided into 7 groups ,8 cases per group . The one side of knee joint was randomly selected for performing the anterior cruciate ligment transection (ACLT) and establishing the OA model .The rats in one group were randomly killed on the day of operation and at postoperative 0 ,2 ,4 ,8 ,12 ,16 ,20 weeks . The femoral condyle cartilage samples at different time periods in the operated side were collected for conducting safranin O /fast green staining and HE staining .Meanwhile ,the OA pathological grade was made out according to the modified Mankin scale .The expression of aggrecan and YKL-40 in the cartilage with different stages of OA were analyzed by the immunohistochemistry meth-od ,and the status of expression were measured by average optical density (AOD) .The correlation between aggrecan and YKL-40 was analyzed .Results With the aggravation of OA ,the expression of aggrecan was gradually reduced and the expression of YKL-40 was gradually increased .The differences during the early ,middle and late phases of OA had statistical significance (P<0 .05) . The expression of aggrecan was negatively correlated with the expression of YKL-40(P<0 .05) .Conclusion The level of aggrecan is gradually reduced with the aggravation of OA .Aggrecan is negatively correlated with the YKL-40 level ,which may reflect the dedifferentiation degree of joint chondrocyte to some extent .关键词
骨关节炎/关节/软骨/蛋白多糖/YKL-40Key words
osteoarthritis/joints/cartilage/aggrecan/YKL-40引用本文复制引用
陈喜德,宋丽君,魏波,刘军,林颢..骨关节炎SD大鼠关节软骨中蛋白多糖与YKL-40的相关性[J].重庆医学,2014,(10):1214-1217,4.基金项目
广东医学院附属医院青年科研基金资助项目（205019010021）。 （）
