重庆医学Issue(11)：1302-1303,1307,3.DOI:10.3969/j.issn.1671-8348.2014.11.008
羊水 ABH血型物质测定与 ABO 血型基因分型
Detection of amniotic fluid ABH blood group substances and ABO blood type gene classification
摘要
Abstract
Objective To detect amniotic fluid ABH blood group substances and ABO blood group genotype by the polymerase chain reaction with sequence-specific primers(PCR-SSP) to increase the prenatal diagnosis of fetal ABO blood group .Methods 53 pregnant women with gestational age 16 -25 weeks were selected .Amniotic fluid was extracted for detecting ABH blood group substances by the serological indirect agglutinating reaction ;the amniotic fluid cells were separated for extracting DNA .Then the PCR-SSP technique was adopted to analyze the ABO blood group genotypes .Results 16 specimens of amniotic fluid were non-se-creting type phenotype(30 .2% ) and 37 specimens of amniotic fluid were secreting type phenotype (69 .8% );48 specimens of amni-otic fluid were detected out the ABO blood group genotype by the PCR-SSP method .ABO blood group of fetal amniotic fluid cells by the gene identification was consistent to the detection results of amniotic fluid secreting type ABH blood group substances .Con-clusion The PCR-SSP technique can accurately detect the fetal amniotic fluid cells ABO blood group .关键词
ABO血型系统/羊水/细胞/序列特异性引物-聚合酶链反应/ABH血型物质Key words
ABO blood-group system/amniotic fluid/cell/polymerase chain reaction with sequence specific primes/ABH blood group substances引用本文复制引用
陈江,逯心敏,郭渝,胡伟..羊水 ABH血型物质测定与 ABO 血型基因分型[J].重庆医学,2014,(11):1302-1303,1307,3.基金项目
四川省卫生厅资助项目（20100583）；宜宾市科技局资助项目（2010SF011）。 （）
