重庆医学Issue(11)：1338-1340,3.DOI:10.3969/j.issn.1671-8348.2014.11.020
花生根乙醇提取物对大鼠前列腺增生抑制作用及对Bcl-2，Bax 蛋白表达影响的研究
Inhibiting effect of ethanol extract from peanut root on rat prostate hyperplasia and its influence on Bcl-2 and Bax protein
闫学红 1罗晓冰 2卑占宇 2马永超2
作者信息
- 1. 河南省漯河市召陵区人民医院，河南漯河462002
- 2. 漯河医学高等专科学校药学系，河南漯河462002
- 折叠
摘要
Abstract
Objective To explore the inhibitory effect of the ethanol extract from peanut root on the non-castration adult rat prostate hyperplasia induced by testosterone propionate and its mechanism .Methods 60 SD rats were randomly divided into the control ,model and high ,middle and low dose of peanut root ethanol extract treatment groups ,among them ,the control group was normally fed without medication ;the model group was subcutaneously injected by testosterone propionate (TP ,5 mL/kg/d) and simultaneously gavaged subcutaneous injection with peanut root ethanol extract (10 mL/kg/d ,5 mLg/kg/d or 1 mL/kg/d) for suc-cessive 14 d .The rats in various groups were killed on 15 d and their prostate ,spermatophore and testicle tissues were separated and weighed .The ratio of gland/body-weight(mg/g) was calculated .The expressions of Bcl-2 and Bax proteins in the prostate tissues were detected by immunohistochemistry .Results There was statistical difference in the indexes of prostate between the treatment groups(high and middle dose) and the model group(P<0 .01) ,while there was no statistical difference between the low dose group and the model group(P>0 .05) .This result showed that there was a dose-effect relationship between the inhibition effect on rat prostate hyperplasia and the ethanol extracts from peanut root .There was no statistical differences between the model group and the treatment groups in the indexes of testicle ,spermatophore or the change of weight (P>0 .05) .There was statistical difference be-tween the control group or model group and the treatment group in the expression of Bcl-2 or Bax protein(P<0 .05) ,while no sta-tistical difference between the treatment group and the control group (P>0 .05) .Conclusion Ethanol extract from peanut root has good inhibiting effect on prostate hyperplasia and its mechanism could reach the curative effect by regulating the balance relation be-tween apoptosis gene Bcl-2 and Bax protein proportion and promoting apopitosis of benign prostatic hyperplasia .关键词
植物提取物/花生根/前列腺增生/大鼠/细胞凋亡/Bcl-2/BaxKey words
plant extracts/peanut root/prostatic hyperplasia/rats/apoptosis/Bcl-2/Bax引用本文复制引用
闫学红,罗晓冰,卑占宇,马永超..花生根乙醇提取物对大鼠前列腺增生抑制作用及对Bcl-2，Bax 蛋白表达影响的研究[J].重庆医学,2014,(11):1338-1340,3.
