重庆医学Issue(11)：1344-1347,1350,5.DOI:10.3969/j.issn.1671-8348.2014.11.022
U QCRC1对 H9c2心肌细胞耐受缺氧／复氧损伤的影响
Influence of ubiquinol-cytochrome C reductase core protein 1 on hypoxia/reoxygenation injury in cultured H9c2 cardiac myocytes
摘要
Abstract
Objective To construct the recombinant adenovirus vector containing ubiquinol-cytochrome C reductase core protein 1(UQCRC1) and to investigate the protective role of UQCRC1 against hypoxia/reoxygenation injury in H9c2 cardiac myocytes . Methods UQCRC1 gene was obtained from the cDNA library by PCR ,then was double-digested with restriction endonucleases SalⅠand XbaⅠand inserted into pAd Track-CMV .The identified plasmid of pAd Track-UQCRC1 was transfected into BJ 5183 contai-ning pAdEasy-1 .After screening the positive clone ,the plasmid was transfect into 293T cells with liposome to integrate and package the recombinant adenovirus .Finally ,these adenoviruses were transfected into H9c2 cardiac myocytes .The expressions of green fluo-rescence protein(GFP) ,UQCRC1 gene and protein were observed by RT-PCR and Western blot analysis .The cell viability and the LDH release were detect .Results The recombinant adenovirus-UQCRC1 was constructed successfully .The overexpression of UQCRC1 increased the cell viability(P<0 .05) and decreased the LDH release(P<0 .05) from H9C2 cardiac myocytes after suf-fering hypoxia/reoxygenation injury .Conclusion UQCRC1 has the protective effect on hypoxia/reoxygenation injury in H9c2 car-diac myocytes ,and the construction of recombinant adenovirus vector will lay the foundation for further studying the role of UQCRC1 in cardioprotection .关键词
电子传递链复合物Ⅲ/重组腺病毒/心肌再灌注损伤/心肌保护/心肌细胞 ,心脏Key words
electron transport complex Ⅲ/recombinant adenovirus vector/myocardial reperfusion injury/cardioprotection/myo-cy tes ,cardiac引用本文复制引用
易婷婷,李洪,吴潇潇,杨天德..U QCRC1对 H9c2心肌细胞耐受缺氧／复氧损伤的影响[J].重庆医学,2014,(11):1344-1347,1350,5.基金项目
国家自然科学基金资助项目（81070094）；重庆市自然科学基金资助项目（2013c292）。 （）
