重庆医学Issue(12)：1476-1479,4.DOI:10.3969/j.issn.1671-8348.2014.12.023
采用改进的三引物等位基因扩增法对痛风相关SN P位点的分型研究
Research on gout-related SNP genotyping by improved tri-primer allele gene amplification
摘要
Abstract
Objective To improve the tri-primer allele gene amplification for realizing the single nucleotide polymorphisms (SNP) genotyping of the peripheral blood sample .Methods Aiming at the peripheral blood samples with the clinical usual antico-agulation processing by EDTA ,heparin and citrate ,with the locus rs1165205 as the target site ,the buffer solution(YW) suitable for whole blood was prepared and the PCR amplification system and the amplification condition were optimized for realizing the detec-tion of SNP genotyping .Results The genotyping results of locus rs1165205 by improved tri-primer allele gene amplification method were consistent with the results of the Sanger sequencing method ,and the peripheral blood samples treated by different anticoagu-lant were genotyped by the improved tri-primer ASA .Among 80 samples ,various genotypes of locus rs1165205 had no statistical differences in the distribution between the gout population and non-gout population(P= 0 .335) .Conclusion The improved tri-primer allele gene amplification method can be adopted to conduct the rapid genotyping research on gout SNP locus of the peripheral blood samples with the clinical usual anticoagulation processing .关键词
三引物扩增法/等位基因/基因扩增/痛风/多态性 ,单核苷酸Key words
tri-primer amplification/alleles/gene amplification/gout/polymorphism ,single nucleotide引用本文复制引用
郑晨娜,王清瑶,黄玉香,叶桂华,许超尘,杨会勇..采用改进的三引物等位基因扩增法对痛风相关SN P位点的分型研究[J].重庆医学,2014,(12):1476-1479,4.基金项目
福建省教育厅 A 类资助项目（JA11323）；泉州市科技局重点资助项目（2011Z5、2011Z30）。 （）
