重庆医学Issue(12)：1480-1482,1484,4.DOI:10.3969/j.issn.1671-8348.2014.12.024
PEDF对小鼠树突细胞表型及免疫功能影响的实验研究
Effects of PEDF on phenotypic and immunologic function of dentritic cells
摘要
Abstract
Objective To explore the effects of pigmentary epithelium derived factor (PDEF) on the phenotypic and immunologic function of murine-derived dentritic cells(BMDCs) .Methods Mononuclear cells(MNCs) isolated from murine bone marrow were cultured in RPMI1640 medium containing rmGM-CSF and rmIL-4 for 5 d ,and were divided into five groups .MNCs were stimulated for 3 d with either 50 ,100 ,200ng/mL PEDF ,1 μg/mL LPS(positive control) or RPMI1640(negative control) .The expression of CD11c ,CD80 and CD86 on DCs surface were analyzed by the fluorescence activated cell sorting (FCM ) .The ability of PEDF-induced BMDCs to stimulated T cell maturation were determined by the CCK-8 method and the level of IL-12 in the culture supernatant was detected by ELISA .Results The PEDF-treated BMDCs expressed high levels of CD11c ,CD80 and CD86 ,enhanced the immunolog-ical activities of T lymphocyte and its secretion of IL-12 when compared with untreated DCs .Conclusion PEDF can significantly up-regulate the expression of DCs immunological labelled molecule in in vitro cultured murine and increase its immunological com-petence .关键词
色素上皮衍生因子/树突细胞/表型/免疫Key words
pigmentary epithelium derived factor/dendritic cells/phenotype/immunity引用本文复制引用
李婵玉,韩健,韩磊,黄威,朱书力,郑秀惠,郭建新,李力..PEDF对小鼠树突细胞表型及免疫功能影响的实验研究[J].重庆医学,2014,(12):1480-1482,1484,4.基金项目
国家卫生行业科研专项基金资助项目（201002013）。 （）
