重庆医学Issue(13)：1591-1593,3.DOI:10.3969/j.issn.1671-8348.2014.13.021
侗族乙型肝炎中CCR5 mRNA的表达
Expression of CCR5 gene mRNA to hepatitis B virus infection in Dong minority
摘要
Abstract
Objective To investigate the expression of CCR5 gene mRNA in peripheral blood of the population between Con-gj iang Dong minority from Guizhou,and to evaluate the relevance between CCR5 gene and hepatitis B infection.Methods A total of twenty individuals with AHB infection(AHB group,20 cases),thirty individuals with CHB infection(CHB group,32 cases),Fifty healthy controls of peripheral blood were recruited to conduct a case-control study among Dong ethnicity.Total RNA from blood was extracted and purified by the trizol-phenol-chloroform one-step method.Expression of CCR5 mRNA was detected by using SYBR green supermix real-time fluorescent quantitative PCR.Results The relative expression of CCR5 mRNA in AHB infectiong-roup was (1.119 9±0.723 3),the data of CCR5 mRNA in CHB group was (0.582 3±0.273 6),the data of CCR5 mRNA in con-trol group was (0.798 5±0.349 2)respectively.The results showed that the expression level of CCR5 mRNA in AHB group was significantly higher than that of the control group,CCR5 mRNA expression was lower in CHB group than in control group,the ex-pression level of CCR5 mRNA in AHB group was significantly higher than CHB group(P<0.05),However the relative expression of CCR5 mRNA had no correlation with age and gender(P>0.05).Conclusion CCR5 gene is associated with AHB and CHB,there is positive correlation between the expression instensity of CCR5 mRNA and the clearance degrees of HBV possibly.关键词
受体,CCR5/肝炎,乙型/急性病/肝炎,乙型,慢性Key words
receptors,CCR5/hepatitis B/acute disease/hepatitis B,chronic引用本文复制引用
张禾璇,何燕,单可人,官志忠..侗族乙型肝炎中CCR5 mRNA的表达[J].重庆医学,2014,(13):1591-1593,3.基金项目
贵州省科技厅科技计划课题基金资助项目（黔科合 LG［2011］024）。 （）
