重庆医学Issue(13)：1594-1596,3.DOI:10.3969/j.issn.1671-8348.2014.13.022
KAI1蛋白对786-O细胞增殖及凋亡的影响
Experiment research ofKAI1 on the proliferation and apoptosis of human renal cell carcinoma cell line 786-O
摘要
Abstract
Objective To investigate the effects of KAI1 on renal cell carcinoma 786-O cells and apoptosis,study on the effect of HAI1 on 786-O growth inhibition.Methods The 786-O cells were treated with viral vector carried HKAI1 gene.The MTT test was used to evaluate the proliferation inhibition of HAI1 on 786-O cells.The flow cytometry were utilized to observe and detect the apoptosis of 786-O cells induced by KAI1.Results When the amount of lentiviral vector-transfected for MOI-10,could be observed under a fluorescence microscope after transfection tumor cell fluorescence expression,MTT and FCM detection shows that the KAI1 could significantly inhibit the proliferation and induce apoptosis of 786-O cells.Conclusion The proliferation inhibiting effects of KAI1 on human renal cell carcinoma cell line 786-O may relat to the apoptosis.关键词
kangai-1蛋白/786-O/细胞增殖/细胞凋亡Key words
kangai-1 protein/786-O cell/proliferation/apoptosis引用本文复制引用
陈峰,鲁伟,窦红珍,游刚,付云锐,谢鑫..KAI1蛋白对786-O细胞增殖及凋亡的影响[J].重庆医学,2014,(13):1594-1596,3.基金项目
重庆市医学科研计划基金资助项目（2010-2-474）。 （）
