重庆医学Issue(15)：1834-1836,3.DOI:10.3969/j.issn.1671-8348.2014.15.004
肺癌患者外周血 AKAP12基因甲基化水平的检测及意义
The detection and significance of AKAP12 methylation levels in peripheral blood in patients with lung cancer
廖和和 1张云锋 2吴树强 3李寒春 3金磊 3任宏2
作者信息
- 1. 陕西省核工业215医院肿瘤外科，陕西咸阳712000
- 2. 西安交通大学第一附属医院胸外科，西安710061
- 3. 西安医学院第一附属医院胸外肿瘤科，西安710077
- 折叠
摘要
Abstract
Objective To detect the levels of AKAP12 methylation by methylation‐specific high‐resolution melting curve(MS‐HRM ) in peripheral blood in patients with colorectal cancer and investigate its clinical significance .Methods We used MS‐HRM technology to detect the levels of AKAP12 methylation in peripheral blood in 60 lung cancer patients ,and analyzed the relationship between the levels of AKAP12 methylation and pathological parameters of lung cancer patients .Results 34(56 .7% ) of the 60 lung cancer patients were found to be methylated at the AKAP12 promoter region by MS‐HRM ,the methylation levels of 18 cases ranged between 1% -20% ,14 cases ranged between 20% -60% ,2 cases ranged between 60% -100% .There was no significant differences between the levels of AKAP12 methylation and lung cancer patients′age and gender(P>0 .05) .However ,it was signifi‐cantly higher in the patients with high pathological stage and differentiation degree (P<0 .05) .Conclusion AKAP12 promoter re‐gion methylation was related to tumor progression and malignant degree .关键词
肺肿瘤/A激酶锚定蛋白12/甲基化/甲基化特异-高分辨融解曲线法/外周血Key words
lung neoplasms/A-kinase anchoring protein 12/methylation/MS-HRM/peripheral blood引用本文复制引用
廖和和,张云锋,吴树强,李寒春,金磊,任宏..肺癌患者外周血 AKAP12基因甲基化水平的检测及意义[J].重庆医学,2014,(15):1834-1836,3.
