重庆医学Issue(15)：1904-1906,3.DOI:10.3969/j.issn.1671-8348.2014.15.028
Grp78基因过表达慢病毒载体构建和包装及鉴定
To constructe,package and identificate the lentiviral vector with overexpression gene Grp78
摘要
Abstract
Objective To constructe ,package and identificate the lentiviral vector with overexpression gene Grp78 .Methods We used lentiviral vector and genetic engineering technology to obtain the aim gene fragment and to constructe recombinant plas‐mid .we prepared competent cells and transform the cells .Through positive clone sequencing ,lentivirus was packaged and virus titer was tested .Results Positive cloning sequence comparison results show that the test was passed .Melt curve did not appear mixed peak ,also did not appear abnormal peak broadening .It means that does not appear pollution ,primer dimers and nonspecific amplifi‐cation in the experiments .Conclusion The construction ,packaging and identification of lentiviral vector with over expression gene Grp78 are sucessful .关键词
Grp78基因/慢病毒载体/基因工程Key words
Grp78 gene/lentiviral vector/gene engineering引用本文复制引用
李亚文,徐世元,张庆国,李乐,赖露颖,郑艇,苏娇玲,杨耐梅,李元涛..Grp78基因过表达慢病毒载体构建和包装及鉴定[J].重庆医学,2014,(15):1904-1906,3.基金项目
深圳市科技计划基金资助项目（201203089）。 （）
