重庆医学Issue(16)：1969-1971,1974,4.DOI:10.3969/j.issn.1671-8348.2014.16.001
SIRT1在TNF-α介导的肠上皮屏障破坏中的作用及机制研究
Action machanism of SIRT1 involves TNF-α-induced intestinal epithelial barrier destroy
摘要
Abstract
Objective To observe the influence of silent information regulator factor 1(SIRT1)on TNF-αinduced intestinal epi-thelial Caco-2 cell barrier function destroy and to investigate its molecular machenism.Methods Caco-2 cells were randomly divided into three groups:normal control group (control),TNF-αgroup (TNF-α,100 ng/mL for 24 h)and 100 ng/mL plus 40μm resvera-trol group (TNF-α+Res).Transepithelial electrical resistance (TER)was determined.SIRT1 and the protein expressions of ZO-1 , occludin were examined by using Western blot.Results The relative expression amounts of SIRT1 protein were 0.81 ± 0.02, 0.43±0.04 and 0.60±0.03 respectively.TER of three groups were (154.00±5.00),(97.00±4.00)and(128.00±6.00)Ohm/cm2 respectively.Compared with the control group,the expression of SIRT1 protein was reduced by 47% and TER was decreased by 37.00% in the TNF-αgroup.After resveratrol precondition,TER was increased by 32.00% compared with the TNF-αgroup. The relative expression amounts of ZO-1 and occludin protein in the control group,TNF-αgroup and TNF-α+Res group were (0.62±0.06,0.57±0.03),(0.23±0.05,0.33±0.04)and(0.41±0.03,0.50±0.02)respectively.After TNF-αtreatment,the ex-pressions of ZO-1 and occludin protein were significantly deceased(P<0.05),but the resveratrol precondition could attenuate this phenomenon,compared with TNF-αgroup,the protein expression was increased by 78.00% and 51.00% respectively (P<0.05). Conclusion Under the condition of TNF-αtreatment,the SIRT1 level is decreased,but increasing SIRT1 level could increase the in-testinal tight j unction protein ZO-1 and occludin protein expression,thus alleviate the damage of TNF-αon the epithelial barrier function constituted by Caco-2 cells.关键词
沉默信息调节因子1/上皮/肿瘤坏死因子-α/跨上皮电阻/紧密连接蛋白Key words
silent-information-regulator-factor-1/epithelium/tumor-necrosis-factor-α/transepithelial-electrical-resistance/tight-j-unction-protein引用本文复制引用
马远航,杨松巍,孙礼刚,于敏,孙力华,王文生,杨桦..SIRT1在TNF-α介导的肠上皮屏障破坏中的作用及机制研究[J].重庆医学,2014,(16):1969-1971,1974,4.基金项目
国家自然科学基金重点资助项目（NSFC 81330013）；国家自然科学基金面上资助项目（NSFC 81272078）；国家教育部创新团队资助项目（IRT 13050）。 （）
