重庆医学Issue(16)：2002-2005,4.DOI:10.3969/j.issn.1671-8348.2014.16.012
胰岛素受体基因酪氨酸激酶区单核苷酸多态性与多囊卵巢综合征的相关性
SNPs of insulin receptor gene in patients with PCOS
印存思 1潘维君 1高超 2崔毓桂2
作者信息
- 1. 安徽省马鞍山市妇幼保健院妇产科 243000
- 2. 南京医科大学第一附属医院生殖医学科，南京 210029
- 折叠
摘要
Abstract
Objective To investigate the relation between single nucleotide polymorphism (SNPs)in the tyrosine kinase domain of the insulin receptor gene (INSR)and polycystic ovary syndrome (PCOS).Methods The loci of 3 single nucleotides (+168715 C>T,+176506 C>T and +176525 G>A)in 96 patients with PCOS (PCOS group)and 48 normal women(control group)were performed the sequence analysis by the polymerase chain reaction restriction fragment polymorphism(PCR-RFLP)technology and the PCR product direct sequencing method.Results The polymorphism frequency of+168715 C>T in exon 17 of the INSR in the PCOS group was 63.5%,which was significantly higher than 45.8% in the control (P=0.043),the difference in the non-obese subgroup was more significant(P=0.039).There was polymorphism of+176506 C>T and+176525 G>A at the front 85bp and 56bp of exon 22 of INSR in the two groups.The polymorphism frequency in these two loci were same,but the polymorphism fre-quency in the PCOS group was 64.6%,which was higher than 47.9% in control group without statistical significance(P=0.055). The further grouping comparison found that the polymorphism frequency of two loci in the non-obese subgroup of PCOS patients was significantly higher than that in the control group(P=0.042).Conclusion The SNPs of+168715 C>T,+176506 C>T and+176525 G>A in INSR is possibly correlated with the PCOS pathogenesis,especially the morbidity of the non-obese patients.关键词
多囊卵巢综合征/胰岛素受体基因/单核苷酸多态性/胰岛素抵抗Key words
polycystic-ovary-symdrome/insulin-receptor-gene/single-nucleotide-polymorphis/insulin-resistance引用本文复制引用
印存思,潘维君,高超,崔毓桂..胰岛素受体基因酪氨酸激酶区单核苷酸多态性与多囊卵巢综合征的相关性[J].重庆医学,2014,(16):2002-2005,4.
