重庆医学Issue(17)：2139-2141,3.DOI:10.3969/j.issn.1671-8348.2014.17.011
AEG-1 siRNA对宫颈癌细胞株增殖和凋亡影响的研究
Study on influence of AEG-1 siRNA on proliferation and apoptosis of cervical carcinoma SiHa cells
秦瑞英 1王宏伟 2赵建华 3杨玉新 1刘颖 1任艳芳1
作者信息
- 1. 新乡医学院第一附属医院妇产科，河南卫辉 453100
- 2. 新乡医学院第一附属医院感染疾病科，河南卫辉 453100
- 3. 新乡医学院第一附属医院神经内科，河南卫辉 453100
- 折叠
摘要
Abstract
Objective To investigate the effect of siRNA-down-regulating astrocyte elevated gene-1(AEG-1)on the proliferation and apoptosis of cervical carcinoma cells.Methods The designed and synthesized AEG-1 siRNA was transfected to the cervical car-cinoma SiHa cells.The influence of AEG-1 siRNA on the expression of AEG-1 gene was detected by real-time fluorescent quantita-tive-polymerase chain reaction (RT-PCR);the inhibiting effect of AEG-1 siRNA on the proliferation of cervical cancer SiHa cells was detected by the MTT method;the influence of AEG-1 siRNA on apoptosis and cell cycle of the cervical cancer SiHa cellswas determined by the flow cytometry.Results Compared with the control group,AEG-1 siRNA transfecting human cervical cancer Si-Ha cells could evidently down-regulate the expression of AEG-1 gene with statistical difference (P<0.01);at the same time,the dwn-regulation of AEG-1 gene expression could significantly inhibit the proliferation of the cervical cancer SiHa cells (P<0.01), promote the apoptosis of SiHa cells (P<0.01)and arrest the cell cycle in G0/G1 phase.Conclusion AEG-1 siRNA can down-regulate the expression of AEG-1 gene in the cervical carcinoma SiHa cells,inhibit the proliferation of the cervical carcinoma SiHa cells and promote their apoptosis.关键词
星形细胞上调基因1/宫颈肿瘤/小干扰RNA/细胞凋亡Key words
astrocyte-elevated-gene-1/uterine-cervical-neoplasms/small-interfering-RNA/apoptotic引用本文复制引用
秦瑞英,王宏伟,赵建华,杨玉新,刘颖,任艳芳..AEG-1 siRNA对宫颈癌细胞株增殖和凋亡影响的研究[J].重庆医学,2014,(17):2139-2141,3.
