重庆医学Issue(17)：2167-2169,3.DOI:10.3969/j.issn.1671-8348.2014.17.020
树突状细胞与细胞因子诱导的杀伤细胞抗前列腺癌细胞的免疫效应
Immune effects of DC-CIK cells against prostate cancer cells
匡幼林 1邓远忠 1梁思敏 1苟欣1
作者信息
- 1. 重庆医科大学附属第一医院泌尿外科，重庆 400016
- 折叠
摘要
Abstract
Objective To study the anti-prostate cancer immunological effect of dentritic cells(DC)and cytokine induced killers (CIK)cells after co-culture.Methods Peripheral blood mononuclear cells were isolated from healthy human and used for genera-ting DC with the treatment of recombinant human granulocyte macrophage colony stimulating factor(rhGM-CSF)and recombinant human interleukin-4(rhIL-4)as well producing CIK with interferon-γ(IFN-γ),CD3 and rhIL-2 in vitro,and then the DC and CIK were cocultured together to produce DC-CIK.The experiment was performed with four groups composed of DC group,DC-CIK group,DC-T group and CIK group.ELISA was used to detect the concentration of IL-12 and IFN-γin cultured supernatant,and the flow cytometry (FCM)was used to measure the cell phenotype,including CD3,CD8 and CD56.The lymphocytes cytotoxic activity against prostate cancer cell was analyzed by the CCK-8 way.Results The concentrations of IL-1 2 and IFN-γin the cutural super-matant in the DC-CIK group were (105.14±2.16)pg/mL and (726.28±21.35)pg/mL respectively,which were significantly higher than those in other three groups (P<0.05).Meanwhile,the expression of CD3+/CD8+ and CD3+/CD56+ were up-regula-ted significantly[(60.9±1.28)% and (27.8±1.01)%,P<0.05)].However,there is no statistics difference among the DC-CIK, DC-T and CIK groups in the CD3 expression.The cytotoxicity against prostate cancer cells was (52.31±2.14)% in the DC-CIK group,(11.14±1.02)% in the DC group,(14.19±1.63)% in the DC-T group and (34.43±2.01)% in the CIK group,the differ-ences among them had statistical significance (P<0.05).Conclusion DC-CIK can induce potent immunological effect against pros-tate cancer cells,which provides the experimental and theoretical basis for the immunotherapy of prostate cancer.关键词
前列腺肿瘤/免疫治疗/树突状细胞Key words
prostate-neoplasms/immunotherapy/dendritic-cell引用本文复制引用
匡幼林,邓远忠,梁思敏,苟欣..树突状细胞与细胞因子诱导的杀伤细胞抗前列腺癌细胞的免疫效应[J].重庆医学,2014,(17):2167-2169,3.
