重庆医学Issue(21)：2753-2755,2758,4.DOI:10.3969/j.issn.1671-8348.2014.21.022
紫苏油诱导人乳腺癌细胞系MCF7凋亡的研究
Research about perilla oil-induced human breast cancer cell line MCF 7 apoptosis
摘要
Abstract
Objective To investigate perilla oil on human breast cancer MCF7 cell growth inhibition and induction of apoptosis , and provide a theoretical basis for the development of perilla oil anti-tumor .Methods Breast cancer MCF7 cells were treated with different dilutions of perilla oil to do tumor cell growth inhibition MTT experiment ,to observe the changes in the nuclear morpholo-gy of apoptotic cells with Hoechst 33258 and PI staining and fluorescence microscopy ,and to detect rate of apoptosis and apoptotic peak with flow cytometry .Results Perilla oil inhibited the proliferation on human breast cancer cell line MCF7 with a time-and concentration-dependent manner .Typical apoptotic nuclear morphological changes could be observed with Hoechst 33258 and PI staining under a fluorescence microscope .Detected by flow cytometry ,apoptosis rate was increased with time and concentration . Conclusion Perilla oil can inhibit human breast cancer MCF7 cell proliferation and induce apoptosis ,suggesting that it may be used as an anticancer drug in clinical practice .关键词
乳腺肿瘤/细胞凋亡/细胞增殖/紫苏油Key words
breast neoplasms/apoptosis/cell proliferation/basil oil分类
医药卫生引用本文复制引用
杜雨柔,赵菊梅,张生军,李建,刘敏丽..紫苏油诱导人乳腺癌细胞系MCF7凋亡的研究[J].重庆医学,2014,(21):2753-2755,2758,4.基金项目
陕西省延安市科技局科技计划项目课题（2009KS-06）。 （）
