重庆医学Issue(21)：2756-2758,3.DOI:10.3969/j.issn.1671-8348.2014.21.023
茶黄素在大鼠气道上皮细胞炎症损伤中的作用及可能机制
Effect of theaflavin on LPS-induced inflammatory injury of rat airway epithelial cell and possible mechanism
摘要
Abstract
Objective To study the influence of theaflavin on the expressions of TLR4 and release of TNF-αand IL-6 in primari-ly cultured rat airway epithelial cells .Methods Lipopolysacchride(LPS) was adopted to establish the in vitro inflammatory injury model of rat airway epithelial cell ,the TNF-αand IL-6 secreted by the airway epithelial cells and the TLR4 protein level in the air-way epithelial cells were detected with ELISA and the expressions of TLR4 mRNA was detected with RT-PCR .Results LPS sig-nificantly induced the airway epithelial cells to secrete TNF-αand IL-6 ,and enhanced the TLR4 mRNA and TLR4 protein expres-sion(P<0 .01) .Theaflavin could inhibit LPS induced TNF-αand IL-6 secretion and TLR4 gene expression(P<0 .05) ,which was related with the theaflavin concentration (P<0 .05) .Conclusion In rat airway epithelial cell culture ,the anti-inflammatory effect of theaflavin may be decrease the release of inflammatory cytokines via inhibiting TLR 4 gene expression and LPS/TLR4 signal trans-duction pathway .关键词
肿瘤坏死因子α/白细胞介素-6/脂多糖/茶黄素/TLR4 mRNAKey words
tumor necrosis factor-alpha/interleukin-6/lipopolysaccharide/theaflavin/TLR4 mRNA分类
医药卫生引用本文复制引用
文秀芳,陈霞,邬海桥,周向东..茶黄素在大鼠气道上皮细胞炎症损伤中的作用及可能机制[J].重庆医学,2014,(21):2756-2758,3.基金项目
重庆市科委资助项目（cstc2013jcyjA1062）。 （）
