重庆医学Issue(22)：2841-2844,4.DOI:10.3969/j.issn.1671-8348.2014.22.004
阻断p38丝裂原活化蛋白激酶在细胞空泡形成中的作用
The role of suppression of p38 MAPK in cellular vacuole formation
摘要
Abstract
Objective To investigate the role of the p38 MAPK pathway in the formation of cytoplasmic vacuoles .Methods Af-ter treated with Anisomycin ,SB203580 or SP600125 ,images of HepG2 ,LM3 ,QBC939 ,Hela and A549 cells were recorded by light microscopy and taken at a magnification of 400 × .The effects of anisomycin ,SB203580 and SP600125 on the activity of p38 and JNK were measured by Western blot .LM3 and A549 cells were stained with the ER-tracker red and the lyso-tracker red and subjec-ted to confocal microscopy analysis .Results (1)Anisomycin could abolish cytoplasmic vacuolization of HepG2 cells .(2)p38 MAPK activation was responsible for anisomycin-induced cytoplasmic vacuolization abolishment .(3)p38 MAPK blocking initiated cytoplas-mic vacuoles formation in various cancer cell lines .(4)p38 MAPK blocking-induced cytoplasmic vacuoles disrupted the integrity of endoplasmic reticulum .(5)p38 MAPK blocking reversibly induced cytoplasmic vacuoles formation .Conclusion These observations provide direct evidence for a role of p38 MAPK signaling in regulating the formation of cytoplasmic vacuoles .关键词
细胞空泡/p38丝裂原活化蛋白激酶/内质网/SB203580Key words
cytoplasmic vacuoles/p38 MAPK/endoplasmic reticulum/SB203580分类
医药卫生引用本文复制引用
张春燕,冯春红,敬健雄,段春燕,刘友平,夏先明,李洪,代荣阳,陈绍坤..阻断p38丝裂原活化蛋白激酶在细胞空泡形成中的作用[J].重庆医学,2014,(22):2841-2844,4.基金项目
国家自然科学基金资助项目（81000886）；教育部新世纪优秀人才支持计划（NCET-11-1058）；四川省杰出青年学术技术带头人培育计划（2013JQ0045）；四川省卫生厅资助项目（090193）。 （）
