重庆医学Issue(22)：2900-2902,3.DOI:10.3969/j.issn.1671-8348.2014.22.025
抑制磷酸腺苷激活的蛋白激酶活性增强人鼻咽癌细胞放射敏感性
Suppression of Amp activated protein kinase activity enhance the radiosensitivity of human nasopharyngeal carcinoma cells
摘要
Abstract
Objective To investigate the effect of Amp activated protein kinase(AMPK)activity in radiosensitivity of human na-sopharyngeal carcinoma cells .Methods Human nasopharyngeal carcinoma cells ,CNE-2 ,were treated with AMPK inhibitor ,Com-pound C(CC) ,for 1 h .Then cells were explored in X-ray .The expression of total AMPK (t-AMPK) ,phosphorylation AMPK (p-AMPK) ,and MAPlLC3 were detected by Western blot .The number of autophagosomes were observed and calculated by transmis-sion electron microscope(TEM) .Cells processed with CC were explored in X-ray .MTT assay was used to detect the difference of two groups in cell proliferation .Cell apoptosis were assayed by flow cytometry .Results The expression of p-AMPK in CC group cells were significantly downregulated compared to the negative control group cells (P< 0 .01) ,while no significant change of t-AMPK expression were found(P>0 .05) .The expression of MAPlLC3 and the number of autophagosomes in CC group cells were significantly decreased compared to the control group cells (P<0 .05) .Correspondingly ,the cell proliferation rate in CC group was lower than in control group ,and the percent of apoptosis cells was higher in CC group than in control group (P<0 .05) .Conclusion Suppression of AM PK activity could inhibited autophagy induced by decreasing the degree of p-AM PK ,then enhanced the effect of proliferation inhibition and apoptosis promotion in CNE-2 cells .The AMPK inhibitor ,CC ,can serve as an effective assistant treatment of radiotherapy for nasopharyngeal carcinoma .关键词
AMPK/抑制剂/鼻咽癌/放射敏感性/自噬Key words
AM PK/inhibitor/nasopharyngeal carcinoma/radiosensitivity/autophagy分类
医药卫生引用本文复制引用
卢小迪,朱小东,赵伟,曲颂,梁霞,苏芳,马慧..抑制磷酸腺苷激活的蛋白激酶活性增强人鼻咽癌细胞放射敏感性[J].重庆医学,2014,(22):2900-2902,3.基金项目
国家自然科学基金资助项目（81160285）；广西壮族自治区自然科学基金资助项目（桂科自2010gxnsfa013240）；2012年广西研究生创新计划项目（YCBZ2012017）。 （）
