重庆医学Issue(22)：2903-2906,4.DOI:10.3969/j.issn.1671-8348.2014.22.026
肝细胞生长因子与表皮生长因子联合培养人胆囊上皮细胞的研究
Experimental research of combined hepatocyte growth factor with epidermal growth factor cultured human gallbladder epithelial cell
摘要
Abstract
Objective To establish the method of combined hepatocyte growth factor (HGF) with epidermal growth factor (EGF) cultured human gallbladder epithelial cells(HGBECs) in vitro .Methods The epithelial layer was peeled away from human gallbladder ,epithelial layer were digested with collagenase Ⅳ and scraped repeatedly .HGBECs were isolated and seeded in cell cul-ture plates containing medium supplemented with or without 10 ng/mL EGF or with 10 ng/mL HGF and 10 ng/mL EGF respec-tively .Then the morphologic changes of the cells were observed and taken photos with inverted phase contrast microscope ,and counted number of cells ,MTT assay detected vigor of cells in different groups .Results The number of the HGBECs of the HGF+EGF group was obviously more than the EGF group ,the duration of the HGBECs of the HGF+ EGF group was obviously longer than the EGF group(19 .3 ± 2 .5)d vs .(14 .2 ± 2 .4)d ,P< 0 .05 .And the HGBECs of the group with HGF+ EGF had better cell vigor .Conclusion HGF combines with EGF added to medium can obviously promote the proliferation of HGBECs and prolong the duration and stabilize morphology of HGBECs in vitro .关键词
胆囊/上皮细胞/肝细胞生长因子/表皮生长因子Key words
bile duct/pithelial cells/hepatocyte growth factor/epidermal growth factor分类
医药卫生引用本文复制引用
邓世康,袁珺,王连敏,王滔,邹浩,张小文..肝细胞生长因子与表皮生长因子联合培养人胆囊上皮细胞的研究[J].重庆医学,2014,(22):2903-2906,4.基金项目
国家自然科学基金资助项目（81260084）；云南省科技厅-昆明医科大学联合专项基金资助项目（2011 FB162）。 （）
