重庆医学Issue(22)：2910-2912,3.DOI:10.3969/j.issn.1671-8348.2014.22.028
新生小鼠海马神经元培养及形态学观察的优化方法
Culturing primary hippocampal neurons of neonatal mouse and morphologic observation
摘要
Abstract
Objective To discuss a optimal culture method of primary hippocampal neurons and a more suitable method of mor-phological observation ,and provide basis to the study of synapse in Alzheimer′s Disease .Methods Postnatal 0 -1 days (P0 -1 ) C57BL/6J mice were decollated and bilateral hippocampus were separated .Low level concentration of trypsin and mechanical disso-ciation were adopted .And culture medium without serum was used to culture neurons .After 17 days culturing ,transfected neurons with Green Fluorescent Protein(GFP) by calcium phosphate precipitation ,and then observed neurons and spines by fluorescence mi-croscope .Results The neurons looked good and healthy by using this method .And the axons ,dendrites and spines which were typ-ical structure of neurons were observed clearly after transfected with GFP .Conclusion The cultured hippocampal neurons look good by this method .And the morphological characteristics of neurons and spines are observed much more clearly after transfected GFP by calcium phosphate precipitation .关键词
海马神经元/磷酸钙共沉淀法/GFP转染/树突棘Key words
hippocampal neurons/calcium phosphate precipitation/GFP transfection/spines分类
医药卫生引用本文复制引用
常翔,王奇,方淑环,张玉,闫蓉,屈赵,侯雪芹,苏如玉,张磊,杨从..新生小鼠海马神经元培养及形态学观察的优化方法[J].重庆医学,2014,(22):2910-2912,3.基金项目
国家自然科学基金面上项目（81273817）；教育部高等学校博士学科点专项科研基金（20114425110007）；高等学校博士学科点专项科研基金（20124425120016）；广东省自然基金资助项目（S2012040006514）；广东省科技计划项目（2010A030100009）。 （）
